NKRF Monoclonal antibody, PBS Only

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• NKRF Monoclonal antibody, PBS Only (68230-1-PBS)

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  WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68230-1-Ig (same clone as 68230-1-PBS)

  WB result of NKRF antibody (68230-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKRF transfected HEK-293 cells. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68230-1-Ig (same clone as 68230-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68230-1-Ig (NKRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68230-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NKRF Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68230-1-PBS

Clone No.1H10C8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, Indirect ELISA

Synonyms

ITBA4, NF kappa B repressing factor, NFKB repressing factor, NKRF, NRF, Protein ITBA4, Transcription factor NRF

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Product Information

68230-1-PBS targets NKRF in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6374

  Product name: Recombinant human NKRF protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 345-690 aa of BC068514

  Sequence: NANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

• Full Name: NFKB repressing factor
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC068514
• Gene Symbol: NKRF
• Gene ID (NCBI): 55922
• RRID: AB_2935317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O15226
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NKRF, also named as Protein ITBA4 or Transcription factor NRF, is a 690 amino acid protein, which contains one G-patch domain and one R3H domain. NKRF is Widely and constitutively expressed, with lower level in colon, peripheral blood lymphocytes, lung and kidney. NKRF interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. NKRF is involved in the constitutive silencing of the IFN-β promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity.