NKTR Polyclonal antibody

NKTR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19978-1-AP

Synonyms

NK-TR protein, NK-TR, NK tumor recognition protein, NK TR protein, Natural-killer cells cyclophilin-related protein

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Validation Data Gallery

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  WB analysis using 19978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19978-1-AP (NKTR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19978-1-AP (NKTR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 19978-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19978-1-AP (NKTR antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 19978-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19978-1-AP (NKTR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19978-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19978-1-AP (NKTR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 19978-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19978-1-AP (NKTR antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 19978-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NKTR antibody (19978-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human liver cancer tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19978-1-AP targets NKTR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: natural killer-tumor recognition sequence
• Calculated Molecular Weight: 166 kDa
• Observed Molecular Weight: 166 kDa
• GenBank Accession Number: NM_005385
• Gene Symbol: NKTR
• Gene ID (NCBI): 4820
• RRID: AB_10639365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKTR is a component of a putative tumor-recognition complex. It is involved in the function of NK cells.