NKX1-2 Polyclonal antibody

NKX1-2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55484-1-AP

Synonyms

bB238F13.2, C10orf121, Homeobox protein SAX 1, NK1 homeobox 2, NKX 1.1, NKX1 2, NKX1.1, NKX1-2

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 55484-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55484-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55484-1-AP (NKX1-2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 55484-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55484-1-AP (NKX1-2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 55484-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55484-1-AP (NKX1-2 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55484-1-AP targets NKX1-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NK1 homeobox 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_001146340
• Gene Symbol: NKX1-2
• Gene ID (NCBI): 390010
• RRID: AB_2881347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.