Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human thyroid cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human lung cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66034-1-Ig targets TTF1/NKX2-1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | NK2 homeobox 1 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | NM_003317 |
| Gene Symbol | TTF1 |
| Gene ID (NCBI) | 7080 |
| RRID | AB_11045663 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43699 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TTF1/NKX2-1 antibody 66034-1-Ig | Download protocol |
| IHC protocol for TTF1/NKX2-1 antibody 66034-1-Ig | Download protocol |
| WB protocol for TTF1/NKX2-1 antibody 66034-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nat Commun Intrinsic resistance to RAS inhibitors is driven by dysregulation of KRAS degradation. | ||
Nat Commun Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features | ||
Adv Sci (Weinh) Excessive MYC Orchestrates Macrophages induced Chromatin Remodeling to Sustain Micropapillary-Patterned Malignancy in Lung Adenocarcinoma | ||
Cancer Biol Med SARS-CoV-2 infection in brain tumors and the association with alterations in the tumor immune microenvironment. | ||
Free Radic Biol Med GnIH-induced mitochondrial dysfunction lead to oxidative stress and apoptosis in thyroid follicular cells, causing hypothyroidism. | ||
Br J Cancer Novel transcription factor zinc finger 514 suppresses lung adenocarcinoma progression and enhances cisplatin sensitivity via transcriptional repression of COL1A1. |
