NKX6-1 Polyclonal antibody

NKX6-1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20974-1-AP

Synonyms

Homeobox protein Nkx 6.1, NK6 homeobox 1, NKX6 1, NKX6.1, NKX6-1, NKX6A

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Validation Data Gallery

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  WB analysis of mouse brain using 20974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20974-1-AP (NKX6-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20974-1-AP targets NKX6-1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NK6 homeobox 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: NM_006168
• Gene Symbol: NKX6-1
• Gene ID (NCBI): 4825
• RRID: AB_2918068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NK6 homeobox 1 (NKX6-1) is a potent bifunctional transcription regulator that binds to AT-rich DNA sequences within the promoter region of target genes. NKX6-1 belongs to the class II proteins of neuronal progenitor factors, and the function of it is to enhance proliferation, glucose-stimulated insulin secretion, and survival in mature pancreatic beta-cells, and it is also essential in regional patterning and neuronal fate determination in central nervous system.