NLGN3 Recombinant monoclonal antibody

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• NLGN3 Recombinant monoclonal antibody (87774-2-RR)

• 

  WB analysis using 87774-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87774-2-RR (NLGN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87774-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87774-2-RR (NLGN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

NLGN3 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87774-2-RR

Clone No.253199D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ASPGX1, AUTSX1, HNL3, neuroligin 3, Neuroligin-3

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Tested Applications

• Positive WB detected in: mouse brain tissue, U-87 MG cells, rat brain tissue, U-251 cells, SH-SY5Y cells, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87774-2-RR targets NLGN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5477

  Product name: Recombinant human NLGN3 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 38-709 aa of NM_181303

  Sequence: QAPAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEPPPSWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNEDCLYLNVYVPTEDVKRISKECARKPNKKICRKGGSGAKKQGEDLADNDGDEDEDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVITLNYRVGVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGIGASCVSLLTLSHHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVGCNVLDTVDMVDCLRQKSAKELVEQDIQPARYHVAFGPVIDGDVIPDDPEILMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVVTADLHARYGSPTYFYAFYHHCQSLMKPAWSDAAHGDEVPYVFGVPMVGPTDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKFIHTKANRFEEVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFHYTSTTTKVPPPDTTHSSHITRRPNGKTWSTKRPAISPAYSNENAQGSWNGDQDAGPLLVENPRDYSTELS

• Full Name: neuroligin 3
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 90 kDa, 110 kDa
• GenBank Accession Number: NM_181303
• Gene Symbol: NLGN3
• Gene ID (NCBI): 54413
• RRID: AB_3745747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NZ94-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuroligins (NLGNs) are cell adhesion molecules located on the postsynaptic side of the synapse that interact with their presynaptic partners neurexins to maintain trans-synaptic connection. Three highly homologous neuroligins are abundantly expressed in rodent brain: NLGN1, NLGN2, NLGN3, while NLGN4 is less abundant in rodent brain and less evolutionarily conserved among all NLGNs isoforms. The literature indicates that significantly elevated basal levels of cross-linked NLGN3-positive dimers (sNLGN3, ~300 kDa) can be detected on the synaptic surface of *Fmr1* KO mice. And also Intracellular NLGN3 (iNGLN3 at about 120 kDa) level was increased in basal state Fmr1 KO synaptoneurosomes (PMID: 30056576).