NLN Polyclonal antibody

NLN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14763-1-AP

Synonyms

AGTBP, Angiotensin binding protein, KIAA1226, MEP, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurolysin, mitochondrial, Neurotensin endopeptidase, NLN

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Validation Data Gallery

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  WB analysis of mouse ovary using 14763-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14763-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 14763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14763-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse ovary tissue, HepG2 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14763-1-AP targets NLN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NLN fusion protein Ag6524
• Full Name: neurolysin (metallopeptidase M3 family)
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001644
• Gene Symbol: NLN
• Gene ID (NCBI): 57486
• RRID: AB_2151973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NLN(Neurolysin, mitochondrial) is also named as AGTBP, KIAA1226 and belongs to the peptidase M3 family. It is an enzyme involved in the metabolic inactivation of bioactive peptides and functions as a monomer of molecular mass 78 kDa. It also can be dected the molecular weight between 70 kDa and 85 kDa according to the reports (PMID:10095765, 11092934). The full length protein has a transit peptide with 37 amino acids.