NLN Polyclonal antibody

NLN Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14763-1-AP

Synonyms

AGTBP, Angiotensin binding protein, KIAA1226, MEP, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurolysin, mitochondrial, Neurotensin endopeptidase, NLN



Tested Applications

Positive WB detected inmouse ovary tissue, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14763-1-AP targets NLN in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLN fusion protein Ag6524
Full Name neurolysin (metallopeptidase M3 family)
Calculated molecular weight 81 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC001644
Gene symbol NLN
Gene ID (NCBI) 57486
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NLN(Neurolysin, mitochondrial) is also named as AGTBP, KIAA1226 and belongs to the peptidase M3 family. It is an enzyme involved in the metabolic inactivation of bioactive peptides and functions as a monomer of molecular mass 78 kDa. It also can be dected the molecular weight between 70 kDa and 85 kDa according to the reports (PMID:10095765, 11092934). The full length protein has a transit peptide with 37 amino acids.

Protocols

Product Specific Protocols
WB protocol for NLN antibody 14763-1-APDownload protocol
IHC protocol for NLN antibody 14763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols