NLN Polyclonal antibody, PBS Only

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• NLN Polyclonal antibody, PBS Only (14763-1-PBS)

• 

  WB analysis of mouse ovary using 14763-1-AP (same clone as 14763-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14763-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14763-1-AP (same clone as 14763-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14763-1-AP (same clone as 14763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14763-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14763-1-AP (same clone as 14763-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14763-1-PBS in a different storage buffer formulation.

NLN Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14763-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

AGTBP, Angiotensin binding protein, Angiotensin-binding protein, EC:3.4.24.16, KIAA1226

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Product Information

14763-1-PBS targets NLN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6524

  Product name: Recombinant human NLN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 289-607 aa of BC001644

  Sequence: KVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQVSFFFPPVNLPISFFRKLLDCCQVSAPNRFFQKLNVFKNFIGLLQNQNFPYPVLFHNIFLLGGSSTSERCLLNNSLWKKNKTICKERLQILCFFLLIL

• Full Name: neurolysin (metallopeptidase M3 family)
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC001644
• Gene Symbol: NLN
• Gene ID (NCBI): 57486
• RRID: AB_2151973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BYT8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NLN(Neurolysin, mitochondrial) is also named as AGTBP, KIAA1226 and belongs to the peptidase M3 family. It is an enzyme involved in the metabolic inactivation of bioactive peptides and functions as a monomer of molecular mass 78 kDa. It also can be dected the molecular weight between 70 kDa and 85 kDa according to the reports (PMID:10095765, 11092934). The full length protein has a transit peptide with 37 amino acids.