NLRP1 Polyclonal antibody

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  IHC staining of human pancreas cancer using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12256-1-AP (NLRP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12256-1-AP (NLRP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12256-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12256-1-AP (NLRP1 antibody) at dilution of 1:50 (under 10x lens).

NLRP1 Polyclonal Antibody for IHC, ELISA

Cat No. 12256-1-AP

Publications(17)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, ELISA

Synonyms

KIAA0926, EC:3.4.-.-, DEFCAP, Death effector filament-forming ced-4-like apoptosis protein, Caspase recruitment domain-containing protein 7

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Tested Applications

• Positive IHC detected in: human pancreas cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 4 publications below
• IF: See 4 publications below

Product Information

12256-1-AP targets NLRP1 in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2908

  Product name: Recombinant human NLRP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-146 aa of BC016965

  Sequence: MPLDPYHSVTWGHAQGSHHFVFGELRVRPILESLRDEPDPDPRPSREGPAGRVGALARGGPEPCDAASPPGGASCAPELARPREDKSAQQAKLEGGTRLCCRCPEESRLVPGGAVSPGDHVLEVSGTRGTCGCRPRRHAGPELAHS

• Full Name: NLR family, pyrin domain containing 1
• Calculated Molecular Weight: 155 kDa
• GenBank Accession Number: BC016965
• Gene Symbol: NLRP1
• Gene ID (NCBI): 22861
• RRID: AB_2298504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9C000
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		MBio; Inducible Nitric Oxide Synthase Is a Key Host Factor for Toxoplasma GRA15-Dependent Disruption of the Gamma Interferon-Induced Antiparasitic Human Response.; Authors - Hironori Bando
human	IF	Mol Neurodegener; The NLRP3 and NLRP1 inflammasomes are activated in Alzheimer's disease.; Authors - Marina Saresella
human	IHC	Front Immunol; Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis.; Authors - Qingling Guo
human	IHC	Front Microbiol; Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.; Authors - Pei-Hui Ding
human	IF	Neuroscience; Overexpression of MicroRNA-9a-5p Ameliorates NLRP1 Inflammasome-mediated Ischemic Injury in Rats Following Ischemic Stroke.; Authors - Cao Yuze
human		Inflammation; Aspergillus fumigatus-Stimulated Human Corneal Epithelial Cells Induce Pyroptosis of THP-1 Macrophages by Secreting TSLP.; Authors - Qingshan Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Debanjan (Verified Customer) (08-17-2020)

The band was very clear and of exact molecular weight

• Applications: Western Blot
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human fibroblasts