NLRP10 Polyclonal antibody

• 

  WB analysis using 27345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27345-1-AP (NLRP10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

NLRP10 Polyclonal Antibody for WB, ELISA

Cat No. 27345-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PAN5, Nucleotide-binding oligomerization domain protein 8, NOD8, NALP10, NACHT, LRR and PYD domains-containing protein 10

---

Tested Applications

• Positive WB detected in: Jurkat cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27345-1-AP targets NLRP10 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26402

  Product name: Recombinant human NLRP10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-180 aa of BC104957

  Sequence: MAMAKARKPREALLWALSDLEENDFKKLKFYLRDMTLSEGQPPLARGELEGLIPVDLAELLISKYGEKEAVKVVLKGLKVMNLLELVDQLSHICLHDYREVYREHVRCLEEWQEAGVNGRYNQVLLVAKPSSESPESLACPFPEQELESVTVEALFDSGEKPSLAPSLVVLQGSAGTGKT

• Full Name: NLR family, pyrin domain containing 10
• Calculated Molecular Weight: 655 aa, 75 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC104957
• Gene Symbol: NLRP10
• Gene ID (NCBI): 338322
• RRID: AB_3669596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86W26
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NLRP10 is a member of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). NLRP10 likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5.