NLRP12 Recombinant monoclonal antibody

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  WB analysis using 83123-6-RR

  Mouse liver tissue was subjected to SDS PAGE followed by western blot with 83123-6-RR (NLRP12 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 83123-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83123-6-RR (NLRP12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 83123-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83123-6-RR (NLRP12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83123-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83123-6-RR against Human NLRP12 were performed. The affinity constant is 0.236 nM.

NLRP12 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83123-6-RR

Clone No.241838E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PAN6, NALP12, NACHT, LRR and PYD domains-containing protein 12, Monarch-1, Monarch 1

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83123-6-RR targets NLRP12 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34281

  Product name: Recombinant human NLRP12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 30-140 aa of BC028069

  Sequence: LYLGTATELGEGKIPWGSMEKAGPLEMAQLLITHFGPEEAWRLALSTFERINRKDLWERGQREDLVRDTPPGGPSSLGNQSTCLLEVSLVTPRKDPQETYRDYVRRKFRLM

• Full Name: NLR family, pyrin domain containing 12
• Calculated Molecular Weight: 1061 aa, 120 kDa
• Observed Molecular Weight: 95-110 kDa
• GenBank Accession Number: BC028069
• Gene Symbol: NLRP12
• Gene ID (NCBI): 91662
• RRID: AB_3670832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P59046
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NACHT, LRR and PYD domains-containing protein 12 (NLRP12) is also named as Monarch-1, NALP12, PYPAF7 and RNO. NLRP12 is as an essential cytosolic sensor for heme plus PAMPs-mediated PANoptosis, inflammation, and pathology (PMID: 37267949). NLRP12 drives inflammasome and PANoptosome activation, cell death, and inflammation in response to heme plus PAMPs or TNF. TLR2/4-mediated signaling through IRF1 induced Nlrp12 expression, which led to inflammasome formation to induce maturation of IL-1β and IL-18 (PMID: 37267949). NLRP12, a pyrin-containing NLR protein, is a negative regulator of innate immune activation and type I interferon (IFN-I) production (PMID: 36719379).