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  • KD/KO Validated

NLRP2 Polyclonal antibody

NLRP2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15182-1-AP

Synonyms

CLR19.9, NALP2, NBS1, NLRP2, PAN1, PYPAF2



Tested Applications

Positive WB detected inA549 cells, human lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15182-1-AP targets NLRP2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLRP2 fusion protein Ag7167
Full Name NLR family, pyrin domain containing 2
Calculated Molecular Weight 121 kDa
Observed Molecular Weight 105-110 kDa
GenBank Accession NumberBC001039
Gene Symbol NLRP2
Gene ID (NCBI) 55655
RRIDAB_2298531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NLRP2 antibody 15182-1-APDownload protocol
IHC protocol for NLRP2 antibody 15182-1-APDownload protocol
IP protocol for NLRP2 antibody 15182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Macrophage IL-1β contributes to tumorigenesis through paracrine AIM2 inflammasome activation in the tumor microenvironment

Authors - Zhi Huan Chew
mouseWB,IHC

Brain Behav Immun

Kynurenine regulates NLRP2 inflammasome in astrocytes and its implications in depression.

Authors - Qingyu Zhang
  • KD Validated
humanWB

Eur J Immunol

NLRP2 negatively regulates antiviral immunity by interacting with TBK1.

Authors - Yanqing Yang
  • KD Validated
humanIHC

Front Microbiol

Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.

Authors - Pei-Hui Ding
mouseIHC

Mol Cell Endocrinol

Glucagon-like peptide-1 analogs mitigate neuroinflammation in Alzheimer's disease by suppressing NLRP2 activation in astrocytes

Authors - Mengjun Zhang
mouseWB

J Neuroimmunol

A selective NLRP3 inflammasome inhibitor attenuates behavioral deficits and neuroinflammation in a mouse model of Parkinson's disease.

Authors - Shuxuan Huang