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  • KD/KO Validated

NLRP2 Polyclonal antibody

NLRP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15182-1-AP

Synonyms

CLR19.9, NALP2, NBS1, NLRP2, PAN1, PYPAF2



Tested Applications

Positive WB detected inA549 cells, human lung tissue, mouse lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15182-1-AP targets NLRP2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLRP2 fusion protein Ag7167
Full Name NLR family, pyrin domain containing 2
Calculated molecular weight 121 kDa
Observed molecular weight 105-110 kDa
GenBank accession numberBC001039
Gene symbol NLRP2
Gene ID (NCBI) 55655
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NLRP2 antibody 15182-1-APDownload protocol
IHC protocol for NLRP2 antibody 15182-1-APDownload protocol
IP protocol for NLRP2 antibody 15182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroimmunol

A selective NLRP3 inflammasome inhibitor attenuates behavioral deficits and neuroinflammation in a mouse model of Parkinson's disease.

Authors - Shuxuan Huang
humanIHC

Front Microbiol

Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.

Authors - Pei-Hui Ding
humanWB

Eur J Immunol

NLRP2 negatively regulates antiviral immunity by interacting with TBK1.

Authors - Yanqing Yang
  • KD Validated
mouseWB,IHC

Brain Behav Immun

Kynurenine regulates NLRP2 inflammasome in astrocytes and its implications in depression.

Authors - Qingyu Zhang
  • KD Validated