NLRP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NLRP2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC

Conjugate

Unconjugated

Publications

5

Cat no : 15182-1-AP

Synonyms

CLR19.9, NALP2, NBS1, NLRP2, PAN1, PYPAF2

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Validation Data Gallery

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  WB analysis of A549 using 15182-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15182-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 15182-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 15182-1-AP (NLRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 15182-1-AP

  IP Result of anti-NLRP2 (IP:15182-1-AP, 4ug; Detection:15182-1-AP 1:500) with A549 cells lysate 400ug.

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  IHC staining of human placenta using 15182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15182-1-AP (NLRP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15182-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15182-1-AP (NLRP2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, human lung tissue, mouse lung tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 3 publications below

Product Information

15182-1-AP targets NLRP2 in WB, IP, IHC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NLRP2 fusion protein Ag7167
• Full Name: NLR family, pyrin domain containing 2
• Calculated molecular weight: 121 kDa
• Observed molecular weight: 105-110 kDa
• GenBank accession number: BC001039
• Gene symbol: NLRP2
• Gene ID (NCBI): 55655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC	Brain Behav Immun; Kynurenine regulates NLRP2 inflammasome in astrocytes and its implications in depression.; Authors - Qingyu Zhang ; KD Validated
human	WB	Eur J Immunol; NLRP2 negatively regulates antiviral immunity by interacting with TBK1.; Authors - Yanqing Yang ; KD Validated
human	IHC	Front Microbiol; Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.; Authors - Pei-Hui Ding
mouse	IHC	Mol Cell Endocrinol; Glucagon-like peptide-1 analogs mitigate neuroinflammation in Alzheimer's disease by suppressing NLRP2 activation in astrocytes; Authors - Mengjun Zhang
mouse	WB	J Neuroimmunol; A selective NLRP3 inflammasome inhibitor attenuates behavioral deficits and neuroinflammation in a mouse model of Parkinson's disease.; Authors - Shuxuan Huang