Tested Applications
| Positive WB detected in | K-562 cells, THP-1 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27897-1-AP targets NLRP7 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27465 Product name: Recombinant human NLRP7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-171 aa of BC109125 Sequence: MTSPQLEWTLQTLLEQLNEDELKSFKSLLWAFPLEDVLQKTPWSEVEEADGKKLAEILVNTSSENWIRNATVNILEEMNLTELCKMAKAEMMEDGQVQEIDNPELGDAEEDSELAKPGEKEGWRNSMEKQSLVWKNTFWQGDIDNFHDDVTLRNQRFIPFLNPRTPRKLTP Predict reactive species |
| Full Name | NLR family, pyrin domain containing 7 |
| Calculated Molecular Weight | 112 kDa, 115 kDa, 118 kDa |
| Observed Molecular Weight | 112 kDa |
| GenBank Accession Number | BC109125 |
| Gene Symbol | NLRP7 |
| Gene ID (NCBI) | 199713 |
| RRID | AB_3086005 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WX94 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NLRP7, also called NALP7 or PYPAF3, is a member of the NOD-like receptors (NLR), a family of proteins that plays a crucial role in the innate immune response. NLRP7 has been reported to emerge from NLRP2 gene. Similar to all inflammasomes, NLRP7 contributes to both pro- and an anti-inflammatory processes, depending on whether it functions in an inflammasome-dependent or independent pathway. The function of NLRP7 inflammasome has also been reported to depend on the master regulatory transcription factor protein that controls cellular inflammation, the NF-kB. In addition to its pro- and anti-inflammatory actions, NLRP7 plays a role in restricting intracellular bacterial replication and can also induce inflammasome assembly upon specific stimulation by FSL-1 (diacylated lipoprotein).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NLRP7 antibody 27897-1-AP | Download protocol |
| WB protocol for NLRP7 antibody 27897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NLRP7 Polyclonal antibody (Cat# 27897-1-AP) has 1 total citation. It was cited in Nature Immunology in a study on noncanonical inflammasome activation driven by cytosolic CTH sensing bacterial lipoproteins. The research field is innate immunity and inflammasome biology. The antibody was used for Western blot analysis in human samples.
| Species | Application | Title |
|---|---|---|
Nat Immunol Cytosolic CTH senses bacterial lipoproteins and drives noncanonical inflammasome activation. |
