NME5 Polyclonal antibody

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• NME5 Polyclonal antibody (12923-1-AP)

NME5 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12923-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

IPIA beta, NDK H 5, NDP kinase homolog 5, NME5, Testis specific nm23 homolog

• 

  WB analysis of mouse testis using 12923-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 12923-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human kidney using 12923-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12923-1-AP (NME5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse testis using 12923-1-AP

  IP result of anti-NME5 (IP:12923-1-AP, 4ug; Detection:12923-1-AP 1:500) with mouse testis tissue lysate 3920 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12923-1-AP (NME5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12923-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12923-1-AP (NME5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, human kidney tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12923-1-AP targets NME5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3610

  Product name: Recombinant human NME5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-212 aa of BC026182

  Sequence: MEISMPPPQIYVEKTLAIIKPDIVDKEEEIQDIILRSGFTIVQRRKLRLSPEQCSNFYVEKYGKMFFPNLTAYMSSGPLVAMILARHKAISYWLELLGPNNSLVAKETHPDSLRAIYGTDDLRNALHGSNDFAAAEREIRFMFPEVIVEPIPIGQAAKDYLNLHIMPTLLEGLTELCKQKPADPLIWLADWLLKNNPNKPKLCHHPIVEEPY

• Full Name: non-metastatic cells 5, protein expressed in (nucleoside-diphosphate kinase)
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC026182
• Gene Symbol: NME5
• Gene ID (NCBI): 8382
• RRID: AB_2152973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NME5(Nucleoside diphosphate kinase homolog 5) belongs to the NDK family. Its expression plays a critical role in spermiogenesis by increasing the cellular levels of GPX5 to eliminate reactive oxygen species. NME5 may have specific functions in the phosphotransfer network involved in spermiogenesis and flagellar movement.

Publications

What published studies show

The NME5 Polyclonal antibody (Cat# 12923-1-AP) has 8 citations published in journals including Cell Reports, Current Biology, Cellular and Molecular Life Sciences, Science China Life Sciences, Journal of Hazardous Materials, International Journal of Cancer, and Asian Journal of Andrology. Research fields span cilia and flagella biology (axoneme assembly, sperm motility, flagellum biogenesis), liver fibrosis and iron homeostasis, breast carcinoma biomarker analysis, and asthenozoospermia genetics. Applications cited include WB, IF, and IHC in mouse and human samples.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	J Hazard Mater; TCDD promotes liver fibrosis through disordering systemic and hepatic iron homeostasis.; Authors - Changying Li
mouse	WB,IF	Sci China Life Sci; Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes; Authors - Huan Wu
mouse	WB,IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB,IF	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	IF	Cell Mol Life Sci; CFAP65 is essential for C2a projection integrity in axonemes: implications for organ-specific ciliary dysfunction and infertility; Authors - Jinyi Chen
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma