NMNAT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NMNAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 11399-1-AP

Synonyms

NaMN adenylyltransferase 1, NMNAT1, PNAT 1, PNAT1

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Validation Data Gallery

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  WB analysis of human testis using 11399-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 11399-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11399-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11399-1-AP (NMNAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 11399-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11399-1-AP (NMNAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11399-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11399-1-AP (NMNAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue, mouse testis tissue, pig heart tissue
• Positive IHC detected in: mouse skeletal muscle tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

11399-1-AP targets NMNAT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMNAT1 fusion protein Ag1944
• Full Name: nicotinamide nucleotide adenylyltransferase 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC014943
• Gene Symbol: NMNAT1
• Gene ID (NCBI): 64802
• RRID: AB_10699868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).

Publications

Species	Application	Title
mouse	IHC	J Am Soc Nephrol; Pre-emptive Short-term Nicotinamide Mononucleotide Treatment in a Mouse Model of Diabetic Nephropathy.; Authors - Itaru Yasuda
human,mouse	IF	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li
human	WB	J Proteome Res; Reduced Nicotinamide Mononucleotide (NMNH) Potently Enhances NAD(+) and Suppresses Glycolysis, the TCA Cycle, and Cell Growth; Authors - Yan Liu
mouse	IHC	Sci Rep; Nicotinamide mononucleotide ameliorates adriamycin-induced renal damage by epigenetically suppressing the NMN/NAD consumers mediated by Twist2; Authors - Kazuhiro Hasegawa
mouse	WB	Food Funct; β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice; Authors - Yanrou Gu
mouse,rat	WB	EMBO J; Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function; Authors - Quanjiang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Caitlin (Verified Customer) (09-17-2020)

I used this antibody on neuronal cell lysate for Western blotting. The antibody was able to detect the protein of interest in my lysate (NMNAT3).

• Applications: Western Blot
• Cell Tissue Type: Mouse neurons