Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11399-1-AP targets NMNAT1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1944 Product name: Recombinant human NMNAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-279 aa of BC014943 Sequence: MENSEKTEVVLLACGSFNPITNMHLRLFELAKDYMNGTGRYTVVKGIISPVGDAYKKKGLIPAYHRVIMAELATKNSKWVEVDTWESLQKEWKETLKVLRHHQEKLEASDCDHQQNSPTLERPGRKRKWTETQDSSQKKSLEPKTKAVPKVKLLCGADLLESFAVPNLWKSEDITQIVANYGLICVTRAGNDAQKFIYESDVLWKHRSNIHVVNEWIANDISSTKIRRALRRGQSIRYLVPDLVQEYIEKHNLYSSESEDRNAGVILAPLQRNTAEAKT Predict reactive species |
| Full Name | nicotinamide nucleotide adenylyltransferase 1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 28-32 kDa |
| GenBank Accession Number | BC014943 |
| Gene Symbol | NMNAT1 |
| Gene ID (NCBI) | 64802 |
| RRID | AB_10699868 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NMNAT1 is a member of the nicotinamide-nucleotide adenylyltransferases (NMNATs) which catalyze nicotinamide adenine dinucleotide (NAD) synthesis (PMID: 28445802). NMNAT is a central enzyme in NAD biosynthesis, catalyzing the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PMID: 17402747). NMNAT1 is widely expressed with the highest levels in skeletal muscle, heart, and kidney(PMID: 11027696). Mutations in NMNAT1 have been shown associated with the LCA9 form of the retinal degeneration pathology Leber's congenital amaurosis (PMID: 22842229, 22842230).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NMNAT1 antibody 11399-1-AP | Download protocol |
| WB protocol for NMNAT1 antibody 11399-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NMNAT1 Polyclonal antibody (Cat# 11399-1-AP) has 16 citations across journals including Metabolism, Nat Cardiovasc Res, EMBO J, J Am Soc Nephrol, Biosens Bioelectron, J Hazard Mater, Aging Cell, Food Funct, Int Immunopharmacol, Int J Mol Sci, Sci Rep, J Proteome Res, Exp Cell Res, Hereditas, Onco Targets Ther, and bioRxiv. Research fields span NAD⁺ metabolism, diabetic nephropathy, aortic aneurysm, aging, ferroptosis, glaucoma, liver injury, cancer, and vascular disease.
| Species | Application | Title |
|---|---|---|
Metabolism Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice. | ||
Nat Cardiovasc Res Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm | ||
Biosens Bioelectron Quantitative dynamics of intracellular NMN by genetically encoded biosensor | ||
J Hazard Mater Nicotinamide metabolic dysregulation plays a key role in Benzo[a]pyrene-induced adverse birth outcomes: Evidence from multi-omics profiling, experimental and epidemiological validation. | ||
J Am Soc Nephrol Pre-emptive Short-term Nicotinamide Mononucleotide Treatment in a Mouse Model of Diabetic Nephropathy. | ||
EMBO J Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function |
Reviews
What customers say
The single review reports a 4-star rating for this antibody used in Western blotting on mouse neuronal cell lysate. The user confirmed it successfully detected the target protein NMNAT3 in their sample, indicating reliable performance for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Caitlin (Verified Customer) (09-17-2020) | I used this antibody on neuronal cell lysate for Western blotting. The antibody was able to detect the protein of interest in my lysate (NMNAT3).
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