NMRAL1 Polyclonal antibody

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• NMRAL1 Polyclonal antibody (15765-1-AP)

NMRAL1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15765-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

HSCARG, NmrA-like family domain-containing protein 1, SDR48A1

• 

  WB analysis using 15765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15765-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 15765-1-AP (NMRAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 15765-1-AP

  IP result of anti-NMRAL1 (IP:15765-1-AP, 4ug; Detection:15765-1-AP 1:1500) with Jurkat cells lysate 1560 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15765-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15765-1-AP (NMRAL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15765-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15765-1-AP (NMRAL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, LNCaP cells, MCF-7 cells, mouse brain tissue, mouse heart tissue, mouse spleen tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15765-1-AP targets NMRAL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8401

  Product name: Recombinant human NMRAL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-299 aa of BC007364

  Sequence: MVDKKLVVVFGGTGAQGGSVARTLLEDGTFKVRVVTRNPRKKAAKELRLQGAEVVQGDQDDQVIMELALNGAYATFIVTNYWESCSQEQEVKQGKLLADLARRLGLHYVVYSGLENIKKLTAGRLAAAHFDGKGEVEEYFRDIGVPMTSVRLPCYFENLLSHFLPQKAPDGKSYLLSLPTGDVPMDGMSVSDLGPVVLSLLKMPEKYVGQNIGLSTCRHTAEEYAALLTKHTRKVVHDAKMTPEDYEKLGFPGARDLANMFRFYALRPDRDIELTLRLNPKALTLDQWLEQHKGDFNLL

• Full Name: NmrA-like family domain containing 1
• Calculated Molecular Weight: 299 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC007364
• Gene Symbol: NMRAL1
• Gene ID (NCBI): 57407
• RRID: AB_2153154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HBL8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The NMRAL1 Polyclonal antibody (Cat# 15765-1-AP) has 2 citations. One appeared in Cell Metabolism (2012), studying Stamp2's role in macrophage inflammation and atherosclerosis protection in mice via WB. The other was published in Diabetes, Metabolic Syndrome and Obesity (2022), investigating STAMP2's attenuation of cardiac dysfunction and insulin resistance in diabetic cardiomyopathy in rats via IF. The associated research fields are cardiovascular metabolism and inflammatory disease.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Metab; Stamp2 controls macrophage inflammation through nicotinamide adenine dinucleotide phosphate homeostasis and protects against atherosclerosis.; Authors - Henrik ten Freyhaus
rat	IF	Diabetes Metab Syndr Obes; STAMP2 Attenuates Cardiac Dysfunction and Insulin Resistance in Diabetic Cardiomyopathy via NMRAL1-Mediated NF-κB Inhibition in Type 2 Diabetic Rats; Authors - Zhan Gao