NMT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NMT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11546-1-AP

Synonyms

N myristoyltransferase 1, Myristoyl-CoA:protein N-myristoyltransferase 1, HsNMT1, Glycylpeptide N-tetradecanoyltransferase 1, EC:2.3.1.97

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Validation Data Gallery

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  WB analysis of SKOV-3 using 11546-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11546-1-AP

  WB result of NMT1 antibody (11546-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMT1 transfected HeLa cells.

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  WB analysis of PC-3 using 11546-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11546-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11546-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11546-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11546-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11546-1-AP (NMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11546-1-AP

  IP result of anti-NMT1 (IP:11546-1-AP, 4ug; Detection:11546-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human gliomas using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11546-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11546-1-AP (NMT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 11546-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NMT1 antibody (11546-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, PC-3 cells, mouse pancreas tissue, HeLa cells, L02 cells, human kidney tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human gliomas tissue, human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

11546-1-AP targets NMT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMT1 fusion protein Ag2072
• Full Name: N-myristoyltransferase 1
• Calculated Molecular Weight: 496 aa, 57 kDa
• Observed Molecular Weight: 49-68 kDa
• GenBank Accession Number: BC006569
• Gene Symbol: NMT1
• Gene ID (NCBI): 4836
• RRID: AB_2153157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.

Publications

Species	Application	Title
human	WB	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič
human	WB,IF	Cancer Lett; N-myristoyltransferase-1 deficiency blocks myristoylation of LAMTOR1 and inhibits bladder cancer progression.; Authors - Yi Sun ; KD Validated
human	WB,IHC	Cell Death Dis; NMT1 inhibition modulates breast cancer progression through stress-triggered JNK pathway.; Authors - Lu Deng ; KD Validated
human	WB	FASEB J; Regulation of co- and post-translational myristoylation of proteins during apoptosis: interplay of N-myristoyltransferases and caspases.; Authors - Perinpanayagam Maneka A MA
mouse	WB	Biochem Biophys Res Commun; N-myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum.; Authors - Elzbieta Dudek
human		Oncotarget; Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model.; Authors - Begoña Díaz