NMUR1 Polyclonal antibody

NMUR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14619-1-AP

Synonyms

FM 3, FM3, G protein coupled receptor 66, GPC R, GPR66, neuromedin U receptor 1, NMUR1

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Validation Data Gallery

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  WB analysis of human colon using 14619-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14619-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 14619-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14619-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human colon tissue, human stomach tissue, mouse pancreas tissue
• Positive IHC detected in: mouse brain tissue, human colon tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14619-1-AP targets NMUR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NMUR1 fusion protein Ag6235
• Full Name: neuromedin U receptor 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC036543
• Gene Symbol: NMUR1
• Gene ID (NCBI): 10316
• RRID: AB_2267310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuromedin-U (NMU) is a widely expressed peptide with the highest abundance in the central nervous system (CNS) and intestinal tract of nearly all vertebrate species. NMU mediates several physiological functions via its two cognate receptors, NMUR1 and NMUR2 (PMID:37102164). NMUR1(NmU receptor 1) has the highest expression in peripheral tissues, particularly the GI tract, pancreas, uterus, and testes but has also been reported in peripheral nerve terminals (PMID:10783389).