NOD2 Polyclonal antibody

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  IHC staining of human colon using 20980-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20980-1-AP (NOD2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 20980-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20980-1-AP (NOD2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 20980-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 20980-1-AP (NOD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

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  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

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  IF Staining using 20980-1-AP

  IF result of 20980-1-AP from publication with PMID: 31186034.

• Featured Product
• KD/KO Validated

NOD2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 20980-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15, CARD15

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Tested Applications

• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 8 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

20980-1-AP targets NOD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: nucleotide-binding oligomerization domain containing 2
• Calculated Molecular Weight: 115 kDa
• GenBank Accession Number: NM_022162
• Gene Symbol: NOD2
• Gene ID (NCBI): 64127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HC29
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS). The antibody is specific to NOD2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC	Kidney Int; PMID: 23594678; Authors - Du Pengchao P
mouse	WB	Kidney Int; Progranulin protects against renal ischemia/reperfusion injury in mice.; Authors - Meng Zhou
mouse	WB	Hypertension; Novel Role of NOD2 in Mediating Ca2+ Signaling: Evidence From NOD2-Regulated Podocyte TRPC6 Channels in Hyperhomocysteinemia.; Authors - Han Huirong H
mouse	WB,IF	J Neuroinflammation; NOD2 promotes dopaminergic degeneration regulated by NADPH oxidase 2 in 6-hydroxydopamine model of Parkinson's disease.; Authors - Li Cheng
rat, human	WB, IHC	Free Radic Biol Med; Identification of NOD2 as a novel target of RNA-binding protein HuR:Evidence from NADPH oxidase-mediated HuR signaling in diabetic nephropathy.; Authors - Jin Shang
human	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Effects of peptidoglycan on the development of steatohepatitis.; Authors - Meiling Jin