NOD2 Antibody

NOD2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A10D1

Cat no : 66710-1-Ig

Synonyms

ACUG, BLAU, CARD15, CD, CLR16.3, IBD1, NLRC2, NOD2, NOD2B, PSORAS1



Tested Applications

Positive WB detected inJurkat cells, THP-1 cells, K-562 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Sample-dependent, check data in validation data gallery

Product Information

66710-1-Ig targets NOD2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name nucleotide-binding oligomerization domain containing 2
Calculated molecular weight 115 kDa
Observed molecular weight 110 kDa
GenBank accession number NM_022162
Gene symbol NOD2
Gene ID (NCBI) 64127
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS).

Protocols

Product Specific Protocols
WB protocol for NOD2 antibody 66710-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Mol Med

β-arrestin 2 negatively regulates NOD2 signalling pathway through association with TRAF6 in microglia after cerebral ischaemia/reperfusion injury.

Authors - Lin Chen
mouseWB,IF

Front Cell Infect Microbiol

Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways.

Authors - Chun-Xia Shi