Tested Applications
| Positive WB detected in | Jurkat cells, THP-1 cells, K-562 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66710-1-Ig targets NOD2 in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nucleotide-binding oligomerization domain containing 2 |
| Calculated Molecular Weight | 115 kDa |
| GenBank Accession Number | NM_022162 |
| Gene Symbol | NOD2 |
| Gene ID (NCBI) | 64127 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HC29 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OD2, also named as CARD15 and IBD1, induces NF-kappa-B via RICK (CARDIAK, RIP2) and IKK-gamma. It confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Defects in NOD2 are the cause of Blau syndrome (BS). Defects in NOD2 are a cause of susceptibility to Crohn disease (CD). Defects in NOD2 are a cause of susceptibility to ulcerative colitis. Defects in NOD2 are the cause of early-onset sarcoidosis (EOS).
Publications
What published studies show
The NOD2 Monoclonal antibody (1A10D1), catalog number 66710-1-Ig, has 4 total citations. These appear in Frontiers in Cellular and Infection Microbiology, Frontiers in Pharmacology (two papers), and Journal of Cellular and Molecular Medicine. The associated research fields include sepsis and pyroptosis, intestinal inflammation and autophagy, toxin-induced NF-κB/MAPK signaling, and NOD2 pathway regulation in cerebral ischaemia/reperfusion injury.
| Species | Application | Title |
|---|---|---|
Front Cell Infect Microbiol Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways. | ||
Front Pharmacol CircEpc1 Promotes Ricin Toxin-Induced Inflammation via Activation of NF-κB and MAPK Signaling Pathways by Sponging miR-5114. | ||
Front Pharmacol Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cells | ||
J Cell Mol Med β-arrestin 2 negatively regulates NOD2 signalling pathway through association with TRAF6 in microglia after cerebral ischaemia/reperfusion injury. |
