Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17613-1-AP targets NOL11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11783 Product name: Recombinant human NOL11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC064404 Sequence: MAALEEEFTLSSVVLSAGPEGLLGVEQSDKTDQFLVTDSGRTVILYKVSDQKPLGSWSVKQGQIITCPAVCNFQTGEYVVVHDNKVLRIWNNEDVNLDKVFKATLSAEVYRILSVQGTEPLVLFKEGAVRGLEALLADPQQKIETVISDEEVIKWTKFFVVFRHPVLIFITEKHGNYFAYVQMFNSRILTKYTLLLGQDENSVIKSFTASVDRKFISLMSLSSDGCIYETLIPIRPADPEKNQSLVKSLLLKAVVSGNARNGVALTALDQDHVAVLGSPLAASKECLSVWNIKFQTLQTSKELPQGTSGQLWYYGEHLFMLHGKSLTVIPYKCEVSSLAGALGKLKHSQDP Predict reactive species |
| Full Name | nucleolar protein 11 |
| Calculated Molecular Weight | 719 aa, 81 kDa |
| Observed Molecular Weight | 75-81 kDa |
| GenBank Accession Number | BC064404 |
| Gene Symbol | NOL11 |
| Gene ID (NCBI) | 25926 |
| RRID | AB_3085529 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H8H0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NOL11, also named as L14, is a ribosome biogenesis factor. It is required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b. NOL11 forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. NOL11 plays a role in pathogenesis of NAIC. There're some isoforms with MW 81 kDa and 19 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOL11 antibody 17613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NOL11 Polyclonal antibody (Cat# 17613-1-AP) has 1 total citation. It was used in a 2025 study published in Cell Mol Biol Lett (IF 12.2), where the antibody was applied by Western blot in human samples. The research focuses on gastric cancer, specifically how the cancer-testis lncRNA LINC01940 drives malignant progression and chemoresistance through TAF15-mediated NOL11 SUMOylation and enhanced ribosome biogenesis.
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett The cancer-testis lncRNA LINC01940 promotes gastric cancer malignant progression and chemoresistance by enhancing ribosome biogenesis via TAF15-mediated NOL11 SUMOylation. |
Reviews
What customers say
One reviewer used this antibody for Western Blot at a 1:1000 dilution on T-cells and reported detecting a weak band at 75 kDa, rating the product 4 out of 5 stars. Overall, the feedback suggests the antibody works but with modest signal intensity under the tested conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wojciech (Verified Customer) (11-05-2025) | Detected a weak band at 75 kDa in T-cells (1:1000).
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