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NOL4 Polyclonal antibody

NOL4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

HRIHFB2255, NOL4, NOLP, Nucleolar localized protein, nucleolar protein 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

-/ -

Freight/Packing: -

Quantity

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What's the best dilution for NOL4 in ?

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14802-1-AP targets NOL4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6518

Product name: Recombinant human NOL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 73-422 aa of BC000313

Sequence: MIDYNMPITMAYMKHMKLQLLNSQQDEDESSIESDEFDMSDSTRMSAVNSDLSSNLEERMQSPQNLHGQQDDDSAAESFNGNETLGHSSIASGGTHSREMGDSNSDGKTGLEQDEQPLNLSDSPLSAQLTSEYRIDDHNSNGKNKYKNLLISDLKMEREARENGSKSPAHSYSSYDSGKNESVDRGAEDLSLNRGDEDEDDHEDHDDSEKVNETDGVEAERLKAFNDESAPADKQCKPEATQATYSTSAVPGSQDVLYINGNGTYSYHSYRGLGGGLLNLNDASSSGPTDLSMKRQLATSSGSSSSSNSRPQLSPTEINAVRQLVAGYRESAAFLLRSADELENLILQQN

Predict reactive species
Full Name nucleolar protein 4
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberBC000313
Gene Symbol NOL4
Gene ID (NCBI) 8715
RRIDAB_2878079
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO94818
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NOL4 antibody 14802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The NOL4 Polyclonal antibody (Cat# 14802-1-AP) has 1 total citation. It appears in the International Journal of Molecular Medicine (IF 8.5), published in November 2017. The study investigated how a type 2 diabetes-associated SNP in KCNQ1 modulates transcription factor binding and CDKN1C expression, with the antibody used for Western blot in rat samples. The research field encompasses molecular medicine, diabetes genetics, and gene regulation.

SpeciesApplicationTitle
ratWB

Int J Mol Med

A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression.

Authors - Masaki Hiramoto
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