NOL4 Polyclonal antibody

NOL4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14802-1-AP

Synonyms

HRIHFB2255, NOL4, NOLP, Nucleolar localized protein, nucleolar protein 4

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Validation Data Gallery

• 

  WB analysis of mouse brain using 14802-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14802-1-AP (NOL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14802-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14802-1-AP (NOL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14802-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14802-1-AP (NOL4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14802-1-AP targets NOL4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOL4 fusion protein Ag6518
• Full Name: nucleolar protein 4
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC000313
• Gene Symbol: NOL4
• Gene ID (NCBI): 8715
• RRID: AB_2878079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Int J Mol Med; A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression.; Authors - Masaki Hiramoto