NOL6 Polyclonal antibody

NOL6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15706-1-AP

Synonyms

NOL6, NRAP, Nucleolar protein 6, UTP22

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Validation Data Gallery

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  WB analysis of SGC-7901 using 15706-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15706-1-AP (NOL6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 15706-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15706-1-AP (NOL6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 15706-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15706-1-AP (NOL6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of PC-3 using 15706-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15706-1-AP (NOL6 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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Tested Applications

• Positive WB detected in: SGC-7901 cells, HeLa cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

15706-1-AP targets NOL6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOL6 fusion protein Ag8378
• Full Name: nucleolar protein family 6 (RNA-associated)
• Calculated Molecular Weight: 1146 aa, 128 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC008298
• Gene Symbol: NOL6
• Gene ID (NCBI): 65083
• RRID: AB_2878169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Cytotechnology; NOL6, a new founding oncogene in human prostate cancer and targeted by miR-590-3p.; Authors - Degang Dong