NOL6 Polyclonal antibody

• Featured Product
• KD/KO Validated

NOL6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16361-1-AP

Synonyms

NOL6, NRAP, Nucleolar protein 6, UTP22

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Validation Data Gallery

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  WB analysis of A431 using 16361-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16361-1-AP (NOL6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16361-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16361-1-AP (NOL6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 16361-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 16361-1-AP (NOL6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

16361-1-AP targets NOL6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOL6 fusion protein Ag8368
• Full Name: nucleolar protein family 6 (RNA-associated)
• Calculated Molecular Weight: 1146 aa, 127 kDa
• Observed Molecular Weight: 127 kDa
• GenBank Accession Number: BC008852
• Gene Symbol: NOL6
• Gene ID (NCBI): 65083
• RRID: AB_2151993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Epigenomics; NOL6 promotes the proliferation and migration of endometrial cancer cells by regulating TWIST1 expression.; Authors - Junhui Liang ; KO Validated