NOL7 Polyclonal antibody

• 

  WB analysis of HEK-293 using 18424-1-AP

  WB result of NOL7 antibody (18424-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOL7 transfected HEK-293 cells.

• 

  WB analysis using 18424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18424-1-AP (NOL7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

NOL7 Polyclonal Antibody for WB, ELISA

Cat No. 18424-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C6orf90, NOP27, Nucleolar protein 7, nucleolar protein 7, 27kDa, Nucleolar protein of 27 kDa

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18424-1-AP targets NOL7 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOL7 fusion protein Ag13272
• Full Name: nucleolar protein 7, 27kDa
• Calculated Molecular Weight: 257 aa, 29 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC023517
• Gene Symbol: NOL7
• Gene ID (NCBI): 51406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UMY1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOL7 (nucleolar protein 7) is localized in both the nucleolus and nucleoplasm, with its nucleolar localization being RNA-dependent. Its function in the nucleolus is likely related to maintaining nucleolar structure and cell growth, while its role in the nucleoplasm may be associated with anti-angiogenic effects. Additionally, NOL7 is involved in the accumulation of early pre-rRNA and the processing of pre-18S rRNA, which are crucial for maintaining nucleolar function and cell growth. The expression of NOL7 is regulated by various transcription factors, including c-myc and RXRα. In cervical cancer, NOL7 transcription is also positively regulated by the RB tumor suppressor gene. The NOL7 gene encodes three distinct splice variants, each with different intracellular localizations that may affect their functions.