NOMO1 Polyclonal antibody

NOMO1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17792-1-AP

Synonyms

NODAL modulator 1, Nomo, NOMO1, PM5, pM5 protein



Tested Applications

Positive WB detected inmouse colon tissue, A431 cells, COLO 320 cells, human brain tissue, mouse brain tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

17792-1-AP targets NOMO1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOMO1 fusion protein Ag11895
Full Name NODAL modulator 1
Calculated molecular weight 1222 aa, 134 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC065535
Gene symbol NOMO1
Gene ID (NCBI) 23420
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NOMO1 antibody 17792-1-APDownload protocol
IHC protocol for NOMO1 antibody 17792-1-APDownload protocol
IF protocol for NOMO1 antibody 17792-1-APDownload protocol
IP protocol for NOMO1 antibody 17792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Sci (Lond)

DLX3 promotes bone marrow mesenchymal stem cell proliferation through H19/miR-675 axis.

Authors - Na Zhao