NOMO2 Polyclonal antibody

NOMO2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14328-1-AP

Synonyms

NODAL modulator 2, Nomo, NOMO2, PM5, pM5 protein 2



Tested Applications

Positive WB detected inBxPC-3 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14328-1-AP targets NOMO2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOMO2 fusion protein Ag5595
Full Name NODAL modulator 2
Calculated Molecular Weight 139 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC041131
Gene Symbol NOMO2
Gene ID (NCBI) 283820
RRIDAB_2152154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NOMO2 antibody 14328-1-APDownload protocol
IHC protocol for NOMO2 antibody 14328-1-APDownload protocol
IF protocol for NOMO2 antibody 14328-1-APDownload protocol
IP protocol for NOMO2 antibody 14328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Ther

Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells.

Authors - Hong Shik Yun