NOMO2 Polyclonal antibody

NOMO2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14328-1-AP

Synonyms

NODAL modulator 2, Nomo, NOMO2, PM5, pM5 protein 2



Tested Applications

Positive WB detected inBxPC-3 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14328-1-AP targets NOMO2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOMO2 fusion protein Ag5595
Full Name NODAL modulator 2
Calculated molecular weight 139 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC041131
Gene symbol NOMO2
Gene ID (NCBI) 283820
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NOMO2 antibody 14328-1-APDownload protocol
IHC protocol for NOMO2 antibody 14328-1-APDownload protocol
IF protocol for NOMO2 antibody 14328-1-APDownload protocol
IP protocol for NOMO2 antibody 14328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Ther

Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells.

Authors - Hong Shik Yun