NOMO2 Polyclonal antibody

NOMO2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14328-1-AP

Synonyms

pM5 protein 2, Nodal modulator 2, BOS complex subunit NOMO2

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Validation Data Gallery

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  WB analysis of BxPC-3 using 14328-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14328-1-AP (NOMO2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14328-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14328-1-AP (NOMO2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14328-1-AP

  IP result of anti-NOMO2 (IP:14328-1-AP, 4ug; Detection:14328-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

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  IHC staining of human pancreas cancer using 14328-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14328-1-AP (NOMO2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14328-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14328-1-AP (NOMO2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of BxPC-3 using 14328-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 14328-1-AP (NOMO2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14328-1-AP targets NOMO2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOMO2 fusion protein Ag5595
• Full Name: NODAL modulator 2
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC041131
• Gene Symbol: NOMO2
• Gene ID (NCBI): 283820
• RRID: AB_2152154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Biol Ther; Radiotherapy diagnostic biomarkers in radioresistant human H460 lung cancer stem-like cells.; Authors - Hong Shik Yun