NOP2 Polyclonal antibody

NOP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10448-1-AP

Synonyms

Nucleolar protein 2 homolog, Nucleolar protein 1, NSUN1, NOP120, NOL1

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Validation Data Gallery

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  WB analysis of A2780 using 10448-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10448-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10448-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10448-1-AP (NOP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10448-1-AP (NOP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10448-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOP2 antibody (10448-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A2780 cells, C6 cells, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10448-1-AP targets NOP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOP2 fusion protein Ag0498
• Full Name: NOP2 nucleolar protein homolog (yeast)
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC000656
• Gene Symbol: NOP2
• Gene ID (NCBI): 4839
• RRID: AB_2282772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13 (PMID: 23775790).

Publications

Species	Application	Title
human	WB,IF	Nucleic Acids Res; DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.; Authors - Calkins Anne S AS
human	WB	Mol Biol Cell; RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S ribosomal subunits.; Authors - Chamousset Delphine D
human	WB	Life Sci Alliance; The nucleolar phase of signal recognition particle assembly; Authors - Amani Issa
human	WB	Transl Cancer Res; Effect of NOP2 knockdown on colon cancer cell proliferation, migration, and invasion.; Authors - Jinling Bi
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi
mouse	IF	Cell Mol Life Sci; N4-acetylcytidine of Nop2 mRNA is required for the transition of morula-to-blastocyst; Authors - Mengyun Wang