NOP56 Polyclonal antibody

NOP56 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18181-1-AP

Synonyms

NOP 56, NOL5A

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 18181-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18181-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18181-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 18181-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18181-1-AP (NOP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 18181-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18181-1-AP (NOP56 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, A549 cells, HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

18181-1-AP targets NOP56 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOP56 fusion protein Ag12882
• Full Name: NOP56 ribonucleoprotein homolog (yeast)
• Calculated Molecular Weight: 594 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC104791
• Gene Symbol: NOP56
• Gene ID (NCBI): 10528
• RRID: AB_2152323
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Box C/D RNA-protein complexes (RNPs) guide the 2'-O-methylation of nucleotides in eukaryotic ribosomal RNAs. The box C/D and C'/D' RNP subcomplexes are each assembled with three sRNP core proteins. The Nop56 core protein mediates crucial protein-protein interactions required for both sRNP assembly and the methyltransferase reaction by bridging the L7Ae and fibrillarin core proteins [PMID:22496443]. NOP56 is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2-prime-O-methylation of pre-ribosomal RNA (rRNA) during its maturation. It is predicted to function in an early to middle step in pre-rRNA processing [PMID:12777385].

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.; Authors - Miao Bai
mouse	WB	iScience; BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.; Authors - Marlene Cervantes