Tested Applications
| Positive WB detected in | HeLa cells, mouse heart tissue, human placenta tissue, mouse liver tissue, mouse lung tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human heart tissue, mouse testis tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20116-1-AP targets NOSTRIN in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13989 Product name: Recombinant human NOSTRIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 122-428 aa of BC014189 Sequence: KWENTLENCYQSILELEKERIQLLCNNLNQYSQHISLFGQTLTTCHTQIHCAISKIDIEKDIQAVMEETAILSTENKSEFLLTDYFEEDPNSAMDKERRKSLLKPKLLRLQRDIEKASKDKEGLERMLKTYSSTSSFSDAKSQKDTAALMDENNLKLDLLEANSYKLSSMLAELEQRPQPSHPCSNSIFRWREKEHTHSYVKISRPFLMKRLENIVSKASSGGQSNPGSSTPAPGAAQLSSRLCKALYSFQARQDDELNLEKGDIVIIHEKKEEGWWFGSLNGKKGHFPAAYVEELPSNAGNTATKA Predict reactive species |
| Full Name | nitric oxide synthase trafficker |
| Calculated Molecular Weight | 506 aa, 58 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC014189 |
| Gene Symbol | NOSTRIN |
| Gene ID (NCBI) | 115677 |
| RRID | AB_10666167 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IVI9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NOSTRIN is classically known as an endothelial cell protein that sequesters eNOS and inhibits NO production. NOSTRIN contains a single polypeptide chain of 506-aa residues of 58 kDa with an N-terminal cdc15 domain and a C-terminal SH3 domain. NOSTRIN mRNA is abundant in highly vascularized tissues such as placenta, kidney, lung, and heart, and NOSTRIN protein is expressed in vascular endothelial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NOSTRIN antibody 20116-1-AP | Download protocol |
| IHC protocol for NOSTRIN antibody 20116-1-AP | Download protocol |
| IP protocol for NOSTRIN antibody 20116-1-AP | Download protocol |
| WB protocol for NOSTRIN antibody 20116-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NOSTRIN Polyclonal antibody (Cat# 20116-1-AP) has 14 citations published in journals including Redox Biology, Cell Communication and Signaling, Environmental Pollution, Ecotoxicology and Environmental Safety, Pharmaceutical Biology, Molecular Medicine Reports, Journal of Inflammation Research, Naunyn-Schmiedeberg's Archives of Pharmacology, Cell Journal, and Translational Andrology and Urology. Research fields span cardiovascular disease (ischemia/reperfusion, hypertension), hepatic oxidative injury, endothelial biology, renal podocyte damage, inflammatory bowel disease, and prostate pathology.
| Species | Application | Title |
|---|---|---|
Redox Biol Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro. | ||
Cell Commun Signal Interleukin 27-induced photoreceptor survival is associated with suppression of a novel Muller glia subpopulation. | ||
Environ Pollut Di-(2-ethylhexyl) phthalate induced an increase in blood pressure via activation of ACE and inhibition of the bradykinin-NO pathway. | ||
Ecotoxicol Environ Saf Comparing the effects of diethylhexyl phthalate and dibutyl phthalate exposure on hypertension in mice. | ||
Pharm Biol Quillaja saponaria bark saponin protects Wistar rats against ferrous sulphate-induced oxidative and inflammatory liver damage. | ||
Mol Med Rep UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats. |
