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NOTCH4 Polyclonal antibody, PBS Only (33280-1-PBS)

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WB analysis using 33280-1-AP (same clone as 33280-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 33280-1-AP (NOTCH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 33280-1-AP (same clone as 33280-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.
IHC staining of mouse lung using 33280-1-AP (same clone as 33280-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 33280-1-AP (same clone as 33280-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 33280-1-AP (same clone as 33280-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 33280-1-AP (same clone as 33280-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 33280-1-AP (same clone as 33280-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.

NOTCH4 Polyclonal antibody, PBS Only

Name: NOTCH4 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 33280-1-PBS

NOTCH4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 33280-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

hNotch4, INT3, Notch 4, Notch 4 extracellular truncation, Notch 4 intracellular domain

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

33280-1-PBS targets NOTCH4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag36738 Product name: Recombinant human NOTCH4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 309-648 aa of BC140782 Sequence: DCSEDVDECETQGPPHCRNGGTCQNSAGSFHCVCVSGWGGTSCEENLDDCIAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHGDAQCSTNPLTGSTLCLCQPGYSGPTCHQDLDECLMAQQGPSPCEHGGSCLNTPGSFNCLCPPGYTGSRCEADHNECLSQPCHPGSTCLDLLATFHCLCPPGLEGQLCEVETNECASAPCLNHADCHDLLNGFQCICLPGFSGTRCEEDIDECRSSPCANGGQCQDQPGAFHCKCLPGFEGPRCQTEVDECLSDPCPVGASCLDLPGAFFCLCPSGFTGQLCEVPLCAPNLCQPKQICKDQKDKANC Predict reactive species
Full Name	Notch homolog 4 (Drosophila)
Calculated Molecular Weight	2003 aa, 210 kDa
Observed Molecular Weight	210 kDa
GenBank Accession Number	BC140782
Gene Symbol	NOTCH4
Gene ID (NCBI)	4855
RRID	AB_3742925
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q99466
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Neurogenic locus notch homolog protein 4 (NOTCH4) is a receptor involved in the Notch signaling pathway, which is synthesized as a precursor that undergoes proteolytic cleavage to form an active receptor. NOTCH4 interacts with various ligands, leading to the release of the Notch intracellular domain (NICD), which translocates to the nucleus and regulates gene expression (PMID: 28794168; 19379690; 17148788). NOTCH4 is involved in various biological processes, including embryonic development, immune cell differentiation, and tumorigenesis (PMID: 24667410; 34925319).