NOVA2 Polyclonal antibody

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NOVA2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 55002-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

ANOVA, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA3, RNA binding protein Nova 2

• 

  WB analysis of mouse brain using 55002-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55002-1-AP (NOVA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 55002-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55002-1-AP (NOVA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 55002-1-AP

  IP result of anti-NOVA2 (IP:55002-1-AP, 4ug; Detection:55002-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55002-1-AP targets NOVA2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: neuro-oncological ventral antigen 2
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 50-55 kDa, 65-70 kDa
• GenBank Accession Number: NM_002516
• Gene Symbol: NOVA2
• Gene ID (NCBI): 4858
• RRID: AB_10858926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UNW9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOVA2 also named as ANOVA and NOVA3. It regulates RNA splicing or metabolism in a specific subset of developing neurons. NOVA2 binds single strand RNA. The antibody is specific to NOVA2. By the alternative splicing, NOVA2 can be detected as 50-55 and 70-80 kDa (PMID:26446569, PMID:9789075).

Publications

What published studies show

The NOVA2 Polyclonal antibody (Cat# 55002-1-AP) has 8 citations published in journals including Neuron, Acta Neuropathologica, EMBO Journal, Hepatology Communications, Nutrients, International Immunopharmacology, Aging, and medRxiv. Research fields span neurodegeneration (ALS), alternative splicing, liver fibrosis, diabetes and obesity, pulmonary fibrosis, neuronal development, and ovarian tumor biology, with applications primarily in Western blot and immunoprecipitation across human and mouse samples.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Neuron; Inhibition of RNA splicing triggers CHMP7 nuclear entry, impacting TDP-43 function and leading to the onset of ALS cellular phenotypes; Authors - Norah Al-Azzam
human	WB	Acta Neuropathol; Aberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis.; Authors - Florian Krach
mouse	WB,IP	EMBO J; Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development.; Authors - Chien-Hsin Chu
human	WB	Hepatol Commun; METTL14 reverses liver fibrosis by inhibiting NOVA2 through an m6A-YTHDF2-dependent mechanism; Authors - Xiaoxue Hou
mouse	WB	Nutrients; Nova1 or Bim Deficiency in Pancreatic β-Cells Does Not Alter Multiple Low-Dose Streptozotocin-Induced Diabetes and Diet-Induced Obesity in Mice; Authors - Manoja K Brahma
mouse,human	WB	Int Immunopharmacol; Melittin-loaded solid lipid nanoparticles inhibit pulmonary fibrosis via the NOVA2-TGF-β/Smads signaling pathway.; Authors - Xinghua Yuan ; KD Validated