NOX1 Polyclonal antibody

NOX1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17772-1-AP

Synonyms

GP91 2, Mitogenic oxidase 1, MOX 1, MOX1, NADPH oxidase 1, NOH 1, NOH1, NOX 1, NOX1



Tested Applications

Positive WB detected inmouse kidney tissue, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17772-1-AP is NOX1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOX1 fusion protein Ag12187
Full Name NADPH oxidase 1
Calculated molecular weight 564 aa, 65 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC075014
Gene symbol NOX1
Gene ID (NCBI) 27035
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NNOX1(NADPH oxidase 1) is also named as MOX1, NOH1 and belongs to the reactive oxygen species (ROS)-generating NADPH oxidase family. It plays a role in host defense, cell growth and differentiation, cell migration, and malignant transformation(PMID:20351171). It also can mediate oncogenic Ras-induced upregulation of VEGF and angiogenesis by activating Sp1 through Ras-ERK-dependent phosphorylation of Sp1(PMID:18454179). It has 3 isoforms produced by alternative splicing with the molecular weight of 65 kDa, 22 kDa and 59 kDa. The full length protein has two glycosylation sites. It always can be detected two bands of 63 kDa and 55 kDa in cells by western blot or another lower band of 45 kDa which is not likely due to alternative translation or splice variation, but as adjustment for the signal peptide and tag removal(PMID:17560373) or unspecifc(PMID:17460729).

Protocols

Product Specific Protocols
WB protocol for NOX1 antibody 17772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Circulation

Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.

Authors - Yu Liming
ratWB

Redox Biol

RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling.

Authors - Nan Wu
mouseWB

Redox Biol

p47phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis.

Authors - Xiamin Wang
mouseWB

PLoS Biol

Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission.

Authors - Dong Ma
humanWB

Antioxid Redox Signal

MCUR1-Mediated Mitochondrial Calcium Signaling Facilitates Cell Survival of Hepatocellular Carcinoma via Reactive Oxygen Species-Dependent P53 Degradation.

Authors - Tingting Ren
mouseWB

J Bone Miner Res

An Antioxidant Sesquiterpene Inhibits Osteoclastogenesis Via Blocking IPMK/TRAF6 and Counteracts OVX-Induced Osteoporosis in Mice.

Authors - Shiyu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (08-23-2022)

I have used this antibody for colon tissue sample and colon cell line like caco2 cells, HT29 cells , it is working excellent.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue sample and colon cell line