Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, HEK-293 cells, PC-12 cells, mouse kidney tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30131-1-AP targets NOX3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32601 Product name: Recombinant human NOX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 223-395 aa of NM_015718 Sequence: GRIVRGQTQDSLSLHNITFCRDRYAEWQTVAQCPVPQFSGKEPSAWKWILGPVVLYACERIIRFWRFQQEVVITKVVSHPSGVLELHMKKRGFKMAPGQYILVQCPAISSLEWHPFTLTSAPQEDFFSVHIRAAGDWTAALLEAFGAEGQALQEPWSLPRLAVDGPFGTALTD Predict reactive species |
| Full Name | NADPH oxidase 3 |
| Calculated Molecular Weight | 64kd |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | NM_015718 |
| Gene Symbol | NOX3 |
| Gene ID (NCBI) | 50508 |
| RRID | AB_2935517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBY0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NOX3 is a member of NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. NOX3 plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. The antibody is specific to NOX3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOX3 antibody 30131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NOX3 Polyclonal antibody (Cat# 30131-1-AP) has 2 published citations. These appear in Life Sciences (2026), studying ASGR1's role in acute liver injury in mice, and Journal of Thoracic and Cardiovascular Surgery (2024), examining cardiac function and angiogenesis in diet-induced metabolic syndrome in pigs. Both studies utilized the antibody for Western blot analysis. The associated research fields encompass hepatology and cardiovascular/metabolic medicine.
| Species | Application | Title |
|---|---|---|
J Thorac Cardiovasc Surg Effects of Diet-induced Metabolic Syndrome on Cardiac Function and Angiogenesis in Response to Sodium-glucose Cotransporter-2 Inhibitor Canagliflozin |
