NOX3-Specific Polyclonal antibody

NOX3-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 20065-1-AP

Synonyms

GP91 3, gp91phox homolog 3, Mitogenic oxidase 2, MOX 2, MOX2, NADPH oxidase 3, NOX3, NOX3-Specific

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Validation Data Gallery

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  WB analysis of human brain using 20065-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20065-1-AP (NOX3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20065-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20065-1-AP (NOX3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

20065-1-AP targets NOX3-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NADPH oxidase 3
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_015718
• Gene Symbol: NOX3
• Gene ID (NCBI): 50508
• RRID: AB_10733223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOX3 is a member of NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. NOX3 plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. The antibody is specific to NOX3.

Publications

Species	Application	Title
mouse	WB	Antioxidants (Basel); Distinction between 2'- and 3'-Phosphate Isomers of a Fluorescent NADPH Analogue Led to Strong Inhibition of Cancer Cells Migration.; Authors - Raoul Manuel
mouse	WB	Front Pharmacol; Nox4 Promotes RANKL-Induced Autophagy and Osteoclastogenesis via Activating ROS/PERK/eIF-2α/ATF4 Pathway; Authors - Jing Sun
human	WB	Onco Targets Ther; Brevilin A Inhibits STAT3 Signaling and Induces ROS-Dependent Apoptosis, Mitochondrial Stress and Endoplasmic Reticulum Stress in MCF-7 Breast Cancer Cells.; Authors - Muhammad Zubair Saleem
human	WB	Biomedicines; Secretome of hESC-Derived MSC-like Immune and Matrix Regulatory Cells Mitigate Pulmonary Fibrosis through Antioxidant and Anti-Inflammatory Effects; Authors - Wenfeng Hu
mouse	WB	J Leukoc Biol; GKT137831 in combination with adipose-derived stem cells alleviates high glucose-induced inflamm-aging and improves diabetic wound healing; Authors - Yunxian Dong
human	WB	Am J Physiol Lung Cell Mol Physiol; Antigen stasis and airway nitrosative stress in human primary ciliary dyskinesia; Authors - Benjamin Gaston