NOXA2/p67phox Recombinant monoclonal antibody

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• NOXA2/p67phox Recombinant monoclonal antibody (88418-1-RR)

NOXA2/p67phox Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88418-1-RR

Clone No.260956B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

NCF2, NOXA2, P67PHOX, NCF 2, NCF-2

• 

  WB analysis using 88418-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88418-1-RR (NCF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of THP-1 using 88418-1-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using NOXA2/p67phox antibody (88418-1-RR, Clone: 260956B9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: THP-1 cells, RAW 264.7 cells, J774A.1 cells, C2C12 cells
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88418-1-RR targets NOXA2/p67phox in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8691

  Product name: Recombinant human NCF2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 179-526 aa of BC001606

  Sequence: VGRLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEQTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV

• Full Name: neutrophil cytosolic factor 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC001606
• Gene Symbol: NCF2
• Gene ID (NCBI): 4688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19878
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.