NPBWR2 Polyclonal antibody

NPBWR2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 24162-1-AP

Synonyms

G protein coupled receptor 8, GPR8, neuropeptides B/W receptor 2, NPBWR2

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Validation Data Gallery

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  IHC staining of human brain using 24162-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24162-1-AP (NPBWR2 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 24162-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24162-1-AP (NPBWR2 Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24162-1-AP is NPBWR2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPBWR2 fusion protein Ag20874
• Full Name: neuropeptides B/W receptor 2
• Calculated Molecular Weight: 333 aa, 37 kDa
• GenBank Accession Number: BC067481
• Gene Symbol: NPBWR2
• Gene ID (NCBI): 2832
• RRID: AB_2879442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPBWR1 (GPR7) and NPBWR2 (GPR8) are two structurally related G-protein coupled receptors. These two receptors share high sequence identity to each other and significant similarity with opioid and somatostatin receptors (PMID: 10407191). Neuropeptide B (NPB) and neuropeptide W (NPW) are endogenous peptide ligands of these receptors. NPBWR1 was found highly conserved in both human and rodents while NPBWR2 gene was not found in the rodent genomes (PMID: 7590751; 10407191). NPBWR1 and NPBWR2 are implicated in energy homeostasis, pain, and emotion (PMID: 23515889).