NPC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NPC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 19888-1-AP

Synonyms

Niemann-Pick disease type C2 protein, Human epididymis-specific protein 1, He1

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Validation Data Gallery

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  WB analysis using 19888-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19888-1-AP (NPC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 19888-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19888-1-AP (NPC2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19888-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19888-1-AP (NPC2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, HT-1080 cells, mouse lung tissue, mouse kidney tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Product Information

19888-1-AP targets NPC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPC2 fusion protein Ag13719
• Full Name: Niemann-Pick disease, type C2
• Calculated Molecular Weight: 151 aa, 17 kDa
• Observed Molecular Weight: 17-21 kDa
• GenBank Accession Number: BC002532
• Gene Symbol: NPC2
• Gene ID (NCBI): 10577
• RRID: AB_10639363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Niemann-Pick Type C (NPC) disease is a lysosomal storage disorder characterized by accumulation of unesterified cholesterol and other lipids in the endolysosomal system. NPC disease results from a defect in either of two distinct cholesterol-binding proteins: a transmembrane protein, NPC1, and a small soluble protein, NPC2. NPC1 or NPC2 deficiency models showed that the function of these two proteins within lysosomes are linked closely. NPC2 is also named human epididymis-specific protein 1 (HE1), defects of which are the cause of Niemann-Pick disease type C2, characterized as a lysosomal storage disorder that affects the viscera and the central nervous system. Recent finding suggests that NPC2 may serve as a novel intracrine/autocrine factor that controls adipocyte differentiation and function as well as potential therapeutic target for the treatment of type 2 diabetes and related metabolic disorders.

Publications

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
human	WB	Dev Cell; NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.; Authors - Oliver B Davis
human	WB	Mol Cell Proteomics; Multi Cell Line Analysis of Lysosomal Proteomes Reveals Unique Features and Novel Lysosomal Proteins; Authors - Fatema Akter
human	WB	Research (Wash D C); Large-Scale CRISPR Screen of LDLR Pathogenic Variants; Authors - Mengjing Li
human,mouse	WB	Metabolism; Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease; Authors - Jiang Du

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kristian (Verified Customer) (07-06-2021)

Paraffin embedded testis were stained for Npc2 (green) and DAPI (blue)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse testis

NPC2 Antibody Immunofluorescence validation (1:200 dilution) in Mouse testis (Cat no:19888-1-AP)