NPEPL1 Polyclonal antibody

NPEPL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17211-1-AP

Synonyms

aminopeptidase like 1, KIAA1974, NPEPL1, Probable aminopeptidase NPEPL1

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Validation Data Gallery

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  WB analysis using 17211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17211-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17211-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17211-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17211-1-AP (NPEPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17211-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17211-1-AP (NPEPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17211-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17211-1-AP (NPEPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 17211-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NPEPL1 antibody (17211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17211-1-AP targets NPEPL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPEPL1 fusion protein Ag10994
• Full Name: aminopeptidase-like 1
• Calculated Molecular Weight: 523 aa, 56 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC020507
• Gene Symbol: NPEPL1
• Gene ID (NCBI): 79716
• RRID: AB_2267625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.