NPHP4 Polyclonal antibody

NPHP4 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, Canine





Cat no : 13812-1-AP


KIAA0673, Nephrocystin 4, nephronophthisis 4, Nephroretinin, NPHP4, SLSN4

Tested Applications

Positive WB detected inmouse brain tissue, Y79 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13812-1-AP targets NPHP4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, Canine samples.

Tested Reactivity human, mouse, Canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPHP4 fusion protein Ag4875
Full Name nephronophthisis 4
Calculated Molecular Weight 1426 aa, 158 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC040520
Gene Symbol NPHP4
Gene ID (NCBI) 261734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPHP4, also named as KIAA0673, is a cilia-associated protein which involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. It is necessary for normal photoreceptor ribbon synapse maintenance and outer segment formation, and for sperm development.


Product Specific Protocols
WB protocol for NPHP4 antibody 13812-1-APDownload protocol
IHC protocol for NPHP4 antibody 13812-1-APDownload protocol
IF protocol for NPHP4 antibody 13812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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