NPHP4 Polyclonal antibody

NPHP4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Canine

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13812-1-AP

Synonyms

KIAA0673, Nephrocystin 4, nephronophthisis 4, Nephroretinin, NPHP4, SLSN4

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Validation Data Gallery

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  WB analysis of mouse brain using 13812-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13812-1-AP (NPHP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13812-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13812-1-AP (NPHP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 13812-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13812-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of hTERT-RPE1 cells using 13812-1-AP

  IF result from Dr. Corbit, Kevin. anti-NPHP4 (13812-1-AP) marks the ciliary axoneme, basal bodies, and the transition zone of Human hTERT-RPE1 cells.

• 

  IF Staining of MDCK using 13812-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Y79 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: hTERT-RPE1 cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 8 publications below

Product Information

13812-1-AP targets NPHP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, Canine samples.

• Tested Reactivity: human, mouse, Canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPHP4 fusion protein Ag4875
• Full Name: nephronophthisis 4
• Calculated molecular weight: 1426 aa, 158 kDa
• Observed molecular weight: 150 kDa
• GenBank accession number: BC040520
• Gene symbol: NPHP4
• Gene ID (NCBI): 261734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPHP4, also named as KIAA0673, is a cilia-associated protein which involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. It is necessary for normal photoreceptor ribbon synapse maintenance and outer segment formation, and for sperm development.

Publications

Species	Application	Title
human,mouse	IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	Nat Cell Biol; Lineage specificity of primary cilia in the mouse embryo.; Authors - Fiona K Bangs
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
mouse	IF	J Cell Biol; The small GTPase RSG1 controls a final step in primary cilia initiation.; Authors - Stephanie O Agbu
human	IF	Genetics; Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.; Authors - Anna Kazatskaya

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