NPHS2 Polyclonal antibody

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  WB analysis of rat kidney using 20384-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20384-1-AP (NPHS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 20384-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20384-1-AP (NPHS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:600 and CoraLite®647-Conjugated Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 20384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using NPHS2 antibody (20384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of zebrafish embryos using 20384-1-AP

  The podocin antibody 20384-1-AP recognized the zebrafish pronephric glomeruli in zebrafish embryos (72 hour-old fish). IF result from Dr. Weibin Zhou.

NPHS2 Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA

Cat No. 20384-1-AP

Publications(58)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (2)

Applications

WB, IHC, IF-P, IF-Fro, ELISA

Synonyms

SRN1, Podocin, PDCN

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Tested Applications

• Positive WB detected in: rat kidney tissue
• Positive IHC detected in: rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue, zebrafish embryos
• Positive IF-Fro detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:300-1:1200
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 37 publications below
• IHC: See 7 publications below
• IF: See 29 publications below

Product Information

20384-1-AP targets NPHS2 in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPHS2 fusion protein Ag14213
• Full Name: nephrosis 2, idiopathic, steroid-resistant (podocin)
• Calculated Molecular Weight: 383 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC029141
• Gene Symbol: NPHS2
• Gene ID (NCBI): 7827
• RRID: AB_10697662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NP85
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPHS2 (also known as Podocin) is a membrane protein located on the podocyte foot process and is the critical component of the glomerular filtration barrier. Mutations of NPHS2 cause recessive steroidresistant nephrotic syndrome. Two isoforms of NPHS2 exist with molecular weights of 42 kDa and 35 kDa, respectively. (PMID: 21499232)

Publications

Species	Application	Title
human	WB,IF	Kidney Int; A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic Nephrotic Syndrome; Authors - Valeryia Kuzmuk
mouse	WB	Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai
rat	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
rat	IF,WB	Front Immunol; Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy; Authors - Xin Lv
mouse	IF	Aging (Albany NY); MiR-320a induces diabetic nephropathy via inhibiting MafB.; Authors - Mengying He
	WB	Cell Physiol Biochem; Weighted Gene Correlation Network Analysis (WGCNA) Detected Loss of MAGI2 Promotes Chronic Kidney Disease (CKD) by Podocyte Damage.; Authors - Zhi Zuo