NPHS2 Polyclonal antibody

NPHS2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (2)

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 20384-1-AP

Synonyms

NPHS2, PDCN, Podocin, SRN1



Tested Applications

Positive WB detected inrat kidney tissue
Positive IF detected inzebrafish embryos

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20384-1-AP is NPHS2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPHS2 fusion protein Ag14213
Full Name nephrosis 2, idiopathic, steroid-resistant (podocin)
Calculated molecular weight 383 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC029141
Gene symbol NPHS2
Gene ID (NCBI) 7827
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NPHS2 (also known as Podocin) is a membrane protein located on the podocyte foot process and is the critical component of the glomerular filtration barrier. Mutations of NPHS2 cause recessive steroidresistant nephrotic syndrome. Two isoforms of NPHS2 exist with molecular weights of 42 kDa and 35 kDa, respectively. (PMID: 21499232)

Protocols

Product Specific Protocols
WB protocol for NPHS2 antibody 20384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Death Dis

Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin.

Authors - Fang Li
mouseIHC,WB

Inflammation

Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.

Authors - Soulixay Senouthai
mouseIF

Am J Physiol Renal Physiol

Expression and Distribution of PIEZO1 in the Mouse Urinary Tract.

Authors - Marianela G Dalghi
rat

Kidney Blood Press Res

Response of Renal Podocytes to Excessive Hydrostatic Pressure: a Pathophysiologic Cascade in a Malignant Hypertension Model.

Authors - Ramzia Abu Hamad
mouseWB,IF

Kidney Blood Press Res

Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5.

Authors - Xu Yang

Methods Mol Biol

A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros.

Authors - Maria Kolatsi-Joannou