NPHS2 Polyclonal antibody
NPHS2 Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 20384-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | rat kidney tissue |
| Positive IF detected in | zebrafish embryos |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 11 publications below |
Product Information
The immunogen of 20384-1-AP is NPHS2 Fusion Protein expressed in E. coli.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NPHS2 fusion protein Ag14213 |
| Full Name | nephrosis 2, idiopathic, steroid-resistant (podocin) |
| Calculated molecular weight | 383 aa, 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC029141 |
| Gene symbol | NPHS2 |
| Gene ID (NCBI) | 7827 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NPHS2 (also known as Podocin) is a membrane protein located on the podocyte foot process and is the critical component of the glomerular filtration barrier. Mutations of NPHS2 cause recessive steroidresistant nephrotic syndrome. Two isoforms of NPHS2 exist with molecular weights of 42 kDa and 35 kDa, respectively. (PMID: 21499232)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NPHS2 antibody 20384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Inhibiting 4E-BP1 re-activation represses podocyte cell cycle re-entry and apoptosis induced by adriamycin. | ||
Inflammation Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model. | ||
Am J Physiol Renal Physiol Expression and Distribution of PIEZO1 in the Mouse Urinary Tract. | ||
Kidney Blood Press Res Response of Renal Podocytes to Excessive Hydrostatic Pressure: a Pathophysiologic Cascade in a Malignant Hypertension Model. | ||
Kidney Blood Press Res Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5. | ||
Methods Mol Biol A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros. |
