B23/NPM1 Polyclonal antibody
B23/NPM1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (2)
Applications
WB, IP, IHC, IF, chIP,ELISA
Conjugate
Unconjugated
Cat no : 10306-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | multi-cells, C6 cells, COLO 320 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
10306-1-AP targets B23/NPM1 in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | B23/NPM1 fusion protein Ag0286 |
| Full Name | nucleophosmin (nucleolar phosphoprotein B23, numatrin) |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC002398 |
| Gene symbol | NPM1 |
| Gene ID (NCBI) | 4869 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for B23/NPM1 antibody 10306-1-AP | Download protocol |
| IHC protocol for B23/NPM1 antibody 10306-1-AP | Download protocol |
| IF protocol for B23/NPM1 antibody 10306-1-AP | Download protocol |
| IP protocol for B23/NPM1 antibody 10306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma. | ||
Signal Transduct Target Ther Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma. | ||
Cell Death Dis Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines. | ||
Int J Mol Sci TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth. | ||
Oncol Lett Protein expression of nucleophosmin, annexin A3 and nm23-H1 correlates with human nasopharyngeal carcinoma radioresistance in vivo. | ||
Virus Genes Subcellular localization of the porcine deltacoronavirus nucleocapsid protein. |
