B23/NPM1 Polyclonal antibody

B23/NPM1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IP, IHC, IF, chIP,ELISA

Conjugate

Unconjugated

Cat no : 10306-1-AP

Synonyms

B23, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin



Tested Applications

Positive WB detected inmulti-cells, C6 cells, COLO 320 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10306-1-AP targets B23/NPM1 in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B23/NPM1 fusion protein Ag0286
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated molecular weight 33 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC002398
Gene symbol NPM1
Gene ID (NCBI) 4869
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Protocols

Product Specific Protocols
WB protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IHC protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IF protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IP protocol for B23/NPM1 antibody 10306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma.

Authors - Haiping Wang
humanIP

Signal Transduct Target Ther

Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma.

Authors - Hang Song
mouseIF

Cell Death Dis

Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines.

Authors - Wei Hu
humanIF

Int J Mol Sci

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

Authors - Ahmed Hassan
mouseIHC

Oncol Lett

Protein expression of nucleophosmin, annexin A3 and nm23-H1 correlates with human nasopharyngeal carcinoma radioresistance in vivo.

Authors - Song Qu
pigIF

Virus Genes

Subcellular localization of the porcine deltacoronavirus nucleocapsid protein.

Authors - Zhen Ding