B23/NPM1 Polyclonal antibody

B23/NPM1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IP, IHC, IF, chIP,ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 10306-1-AP

Synonyms

B23, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

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Validation Data Gallery

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  WB analysis using 10306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23/NPM1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells using 10306-1-AP

  WB result of anti-NPM1 (10306-1-AP) in different cell lysates.

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  WB analysis of K-562 using 10306-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (NPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10306-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (NPM1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10306-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (B23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10306-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (NPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10306-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (NPM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10306-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10306-1-AP (NPM1 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 10306-1-AP

  IP Result of anti-B23 (IP:10306-1-AP, 4ug; Detection:10306-1-AP 1:2000) with Jurkat cells lysate 3200ug.

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  IHC staining of human colon cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10306-1-AP (B23/NPM1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10306-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10306-1-AP (NPM1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10306-1-AP (B23 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: multi-cells, C6 cells, COLO 320 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

10306-1-AP targets B23/NPM1 in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B23/NPM1 fusion protein Ag0286
• Full Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 35-40 kDa
• GenBank accession number: BC002398
• Gene symbol: NPM1
• Gene ID (NCBI): 4869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Publications

Species	Application	Title
human	WB	Mol Med Rep; High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma.; Authors - Haiping Wang
human	IP	Signal Transduct Target Ther; Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma.; Authors - Hang Song
mouse	IF	Cell Death Dis; Nucleolar stress regulation of endometrial receptivity in mouse models and human cell lines.; Authors - Wei Hu
human	IF	Int J Mol Sci; TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.; Authors - Ahmed Hassan
mouse	IHC	Oncol Lett; Protein expression of nucleophosmin, annexin A3 and nm23-H1 correlates with human nasopharyngeal carcinoma radioresistance in vivo.; Authors - Song Qu
pig	IF	Virus Genes; Subcellular localization of the porcine deltacoronavirus nucleocapsid protein.; Authors - Zhen Ding

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