Tested Applications
| Positive WB detected in | COLO 320 cells, Jurkat cells, multi-cells, K-562 cells, HeLa cells, HEK-293 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 25 publications below |
| IHC | See 4 publications below |
| IF | See 16 publications below |
| IP | See 8 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
10306-1-AP targets B23/NPM1 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | B23/NPM1 fusion protein Ag0286 Predict reactive species |
| Full Name | nucleophosmin (nucleolar phosphoprotein B23, numatrin) |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC002398 |
| Gene Symbol | NPM1 |
| Gene ID (NCBI) | 4869 |
| RRID | AB_2155163 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06748 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for B23/NPM1 antibody 10306-1-AP | Download protocol |
| IHC protocol for B23/NPM1 antibody 10306-1-AP | Download protocol |
| IF protocol for B23/NPM1 antibody 10306-1-AP | Download protocol |
| IP protocol for B23/NPM1 antibody 10306-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology. | ||
Cancer Res LNMICC promotes nodal metastasis of cervical cancer by reprogramming fatty acid metabolism. | ||
Int J Mol Sci TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth. | ||
Mol Ther Nucleic Acids RPL32 Promotes Lung Cancer Progression by Facilitating p53 Degradation. | ||
