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What's the best dilution for NPR1 in ?
Tested Applications
| Positive WB detected in | SGC-7901 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55116-1-AP targets NPR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) |
| Calculated Molecular Weight | 119 kDa |
| Observed Molecular Weight | 119 kDa |
| GenBank Accession Number | NM_000906 |
| Gene Symbol | NPR1 |
| Gene ID (NCBI) | 4881 |
| RRID | AB_2881271 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16066 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NPR1 antibody 55116-1-AP | Download protocol |
| IHC protocol for NPR1 antibody 55116-1-AP | Download protocol |
| WB protocol for NPR1 antibody 55116-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
NPR1 Polyclonal antibody (55116-1-AP) has 4 citations across the following journals: J Biol Chem (endothelial H2S release/vascular signaling), J Cardiol (natriuretic peptide-dependent angiogenic effects in cardiomyocytes), Int J Clin Exp Med (NPR-A expression in gastric cancer), and Endocr Connect (ANP inhibitory mechanism on aldosterone production). The research fields span cardiovascular biology, cardiac signaling, oncology, and endocrinology, with human samples used predominantly in WB applications.
| Species | Application | Title |
|---|---|---|
J Biol Chem 17β-Estradiol nongenomically induces vascular endothelial H2S release by promoting phosphorylation of cystathionine γ-lyase. | ||
J Cardiol Generation of ANP- and BNP-deficient human iPSC-derived cardiomyocytes reveals natriuretic peptide-dependent Angiogenic paracrine effects. | ||
Endocr Connect Inhibitory mechanism of ANP on aldosterone production in human adrenocortical cells. |
