NPR1 Recombinant antibody

• 

  WB analysis using 84043-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84043-1-RR (NPR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 84043-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NPR1 antibody (84043-1-RR, Clone: 240696A9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

NPR1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84043-1-RR

Clone No.240696A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GC A, ANPRA, ANPR A, ANPa, ANP A

---

Tested Applications

• Positive WB detected in: HT-29 cells, HeLa cells, SGC-7901 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84043-1-RR targets NPR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 119-125 kDa
• GenBank Accession Number: NM_000906
• Gene Symbol: NPR1
• Gene ID (NCBI): 4881
• RRID: AB_3671610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P16066
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPR1(Atrial natriuretic peptide receptor 1), also named as NPRA , is a transmembrane protein expressed in the vascular endothelium. Activation of NPRA requires a binding of natriuretic peptides NPPA (ANP) or NPPB (BNP) to its extracellular domain and this promotes the production of cyclic guanosine monophosphate (cGMP), which in turn activates protein kinase cGMP‐dependent 1 (PRKG1 or commonly used as PKG).