NPR3 Polyclonal antibody

NPR3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26706-1-AP

Synonyms

ANP C, ANPR C, ANPRC, GUCY2B, NPR3

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Validation Data Gallery

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  IHC staining of human kidney using 26706-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26706-1-AP (NPR3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26706-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26706-1-AP (NPR3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 26706-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26706-1-AP (NPR3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26706-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26706-1-AP (NPR3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 26706-1-AP is NPR3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NPR3 fusion protein Ag19071
• Full Name: natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C)
• Calculated Molecular Weight: 541 aa, 60 kDa
• GenBank Accession Number: BC131540
• Gene Symbol: NPR3
• Gene ID (NCBI): 4883
• RRID: AB_2880607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C), also known as NPR3, is an atrial natriuretic peptide receptor. The family of natriuretic peptides elicit a number of vascular, renal, and endocrine effects that are important in the maintenance of blood pressure and extracellular fluid volume. NPR3 has 3 isoforms with MWs of 37~60 kDa produced by alternative splicing, and may perform higher MW of 70 kDa with phosphorylation.

Publications

Species	Application	Title
human	IHC,IF	Sci Rep; A novel immune score model predicting the prognosis and immunotherapy response of breast cancer; Authors - Wenchang Lv