NPTN Recombinant monoclonal antibody

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• NPTN Recombinant monoclonal antibody (87125-3-RR)

• 

  WB analysis using 87125-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87125-3-RR (NPTN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87125-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87125-3-RR (NPTN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

NPTN Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87125-3-RR

Clone No.252190G4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Neuroplastin, GP55, GP65, np55, np65

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87125-3-RR targets NPTN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5652

  Product name: Recombinant human NPTN protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 29-339 aa of NM_012428.3

  Sequence: QNAGFVKSPMSETKLTGDAFELYCDVVGSPTPEIQWWYAEVNRAESFRQLWDGARKRRVTVNTAYGSNGVSVLRITRLTLEDSGTYECRASNDPKRNDLRQNPSITWIRAQATISVLQKPRIVTSEEVIIRDSPVLPVTLQCNLTSSSHTLTYSYWTKNGVELSATRKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKAAPDITGHKRSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINKENYTELNIVNLQITEDPGEYECNATNAIGSASVVTVLRVRSHLAP

• Full Name: neuroplastin
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_012428.3
• Gene Symbol: NPTN
• Gene ID (NCBI): 27020
• RRID: AB_3745356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y639-2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuroplastin (Nptn) is a member of the Ig superfamily and is expressed in two isoforms, Np55 and Np65. Np65 contains three Ig domains (Ig1, Ig2, Ig3). Np55 contains only two (Ig2, Ig3). Np65 regulates synaptic transmission but the function of Np55 is unknown. Expression of the 65 kD neuroplastin (Np65) isoform derived by alternative splicing from the Nptn gene is restricted to brain neurons, whereas the 55 kD isoform (Np55) is present in most cell types but not in glia. (PMID: 33844052, PMID: 33299977, PMID: 27503893,PMID: 27581460)