Tested Applications
| Positive WB detected in | mouse brain tissue, HepG2 cells, mouse liver tissue, mouse cerebellum tissue, rat cerebellum tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20656-1-AP targets NPTX1 in WB, IHC, IF/ICC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | neuronal pentraxin I |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | NM_002522 |
| Gene Symbol | NPTX1 |
| Gene ID (NCBI) | 4884 |
| RRID | AB_10793669 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15818 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NPTX1, also named as NP1, may mediate uptake of degraded synaptic material which could play an important role in synaptic remodeling. NPTX1 can mediate the neuronal and glial uptake of the snake venom toxin taipoxin. The antibody is specific to NPTX1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NPTX1 antibody 20656-1-AP | Download protocol |
| IHC protocol for NPTX1 antibody 20656-1-AP | Download protocol |
| WB protocol for NPTX1 antibody 20656-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NPTX1 Polyclonal antibody (Cat# 20656-1-AP) has 9 citations published in journals including J Neuroinflammation, Oncogene, Frontiers in Immunology, PLoS Biology, Acta Biochimica et Biophysica Sinica, PLoS ONE, Aesthetic Plastic Surgery, and Cancer Management Research. The research fields span neuroinflammation, hepatocellular carcinoma, synaptic biology, metabolic regulation, melanoma, adipose tissue biology, and gastric cancer.
| Species | Application | Title |
|---|---|---|
J Neuroinflammation Microglia-derived ADAM9 promote GHRH neurons pyroptosis by Mad2L2-JNK-caspase-1 pathway in subarachnoid hemorrhage | ||
Oncogene Chromatin-associated OGT promotes the malignant progression of hepatocellular carcinoma by activating ZNF263 | ||
Front Immunol Identification of Neuronal Pentraxins as Synaptic Binding Partners of C1q and the Involvement of NP1 in Synaptic Pruning in Adult Mice. | ||
PLoS Biol Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling | ||
Acta Biochim Biophys Sin (Shanghai) miR-139-5p mediates the palmitate-induced inhibition of insulin secretion by targeting neuronal pentraxin 1 in INS-1 cells. | ||
PLoS One Exosome-delivered circRPS5 inhibits the progression of melanoma via regulating the miR-151a/NPTX1 axis
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