NPTXR Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85994-1-RR (same clone as 85994-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85994-1-RR (NPTXR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

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  WB analysis using 85994-1-RR (same clone as 85994-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 85994-1-RR (NPTXR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85994-1-RR (same clone as 85994-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85994-1-RR (NPTXR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85994-1-RR (same clone as 85994-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85994-1-RR (NPTXR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 85994-1-RR (same clone as 85994-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NPTXR antibody (85994-1-RR, Clone: 250488B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 85994-1-RR (same clone as 85994-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NPTXR antibody (85994-1-RR, Clone: 250488B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85994-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85994-1-RR against Human NPTXR were performed. The affinity constant is 0.161 nM.

NPTXR Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 85994-1-PBS

Clone No.250488B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Neuronal pentraxin receptor

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Product Information

85994-1-PBS targets NPTXR in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3109

  Product name: Recombinant Human NPTXR protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 24-500 aa of NM_014293.3

  Sequence: ASVPLAASPARALPGGADNASVASGAAASPGPQRSLSALHGAGGSAGPPALPGAPAASAHPLPPGPLFSRFLCTPLAAACPSGAQQGDAAGAAPGEREELLLLQSTAEQLRQTALQQEARIRADQDTIRELTGKLGRCESGLPRGLQGAGPRRDTMADGPWDSPALILELEDAVRALRDRIDRLEQELPARVNLSAAPAPVSAVPTGLHSKMDQLEGQLLAQVLALEKERVALSHSSRRQRQEVEKELDVLQGRVAELEHGSSAYSPPDAFKISIPIRNNYMYARVRKALPELYAFTACMWLRSRSSGTGQGTPFSYSVPGQANEIVLLEAGHEPMELLINDKVAQLPLSLKDNGWHHICIAWTTRDGLWSAYQDGELQGSGENLAAWHPIKPHGILILGQEQDTLGGRFDATQAFVGDIAQFNLWDHALTPAQVLGIANCTAPLLGNVLPWEDKLVEAFGGATKAAFDVCKGRAKA

• Full Name: neuronal pentraxin receptor
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 35-65 kDa
• GenBank Accession Number: NM_014293.3
• Gene Symbol: NPTXR
• Gene ID (NCBI): 23467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95502
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NPTXR (Neuronal Pentraxin Receptor) is a type II transmembrane glycoprotein primarily expressed in the cerebellum and hippocampus. It mediates synaptic plasticity by clustering AMPA-type glutamate receptors at excitatory synapses and promoting synaptogenesis. NPTXR can be proteolytically cleaved to release a soluble form. Its dysregulation is implicated in Alzheimer's disease and small-cell lung cancer.(PMID: 9261167; 18367087; 19368810; 16115696)