NQO1 Recombinant monoclonal antibody

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• NQO1 Recombinant monoclonal antibody (86693-2-RR)

• KD/KO Validated

NQO1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86693-2-RR

Clone No.251713C3

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

Azoreductase, DHQU, DIA 4, DIA4, DT diaphorase

• 

  WB analysis using 86693-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 86693-2-RR

  HSC-T6 cell lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 86693-2-RR

  K-562 cell lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 86693-2-RR

  WB result of NQO1 antibody (86693-2-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NQO1 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 86693-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86693-2-RR against Human NQO1 were performed. The affinity constant is 1.80 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HSC-T6 cells, K-562 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86693-2-RR targets NQO1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2009

  Product name: Recombinant human NQO1 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-274 aa of BC007659

  Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

• Full Name: NAD(P)H dehydrogenase, quinone 1
• Calculated Molecular Weight: 274 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC007659
• Gene Symbol: NQO1
• Gene ID (NCBI): 1728
• RRID: AB_3745057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15559
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640, PMID:18848868)

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Prod Res; Chemical constituents with anti-inflammatory activity from the rhizomes of Amomum sericeum Roxb.; Authors - Wang Luo
mouse	WB	Adv Sci (Weinh); Procyanidin Capsules Combat ALF by Restoring Mitochondrial Homeostasis and Inhibiting Necroptosis via the PGAM5/DRP1/PINK1 Pathway.; Authors - Qing Shi
mouse	WB	Int J Mol Sci; Kadsura coccinea Roots Ameliorated Alcohol-Induced Liver Injury by Modulating Oxidative Stress Through the Regulation of the Nrf2/MAPK Signaling Pathway.; Authors - Yashi Wang
mouse	WB	Int J Biol Macromol; Effects of different deproteinization methods on the hepatoprotective activity of Smilax china L. polysaccharides against CCl(4)-induced acute liver injury in mice.; Authors - Wenjing Wang
mouse	WB	Foods; Chemical Composition, Antioxidant Activity, Anti-Fatigue Function and Mechanism of Pomegranate Peel Polyphenols on Exercise-Induced Fatigue in Mice.; Authors - Xing-Yu Ma
mouse	WB	Pediatr Discov; Therapeutic Effects of Phyllanthus urinaria L on Cisplatin-Induced Acute Kidney Injury: Anti-Inflammatory, Anti-Apoptotic, and Anti-Oxidative Action.; Authors - Qitong Guo