|Positive WB detected in||HeLa cells, mouse liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
15767-1-AP targets NQO2 in WB, IP, IHC, IF applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NQO2 fusion protein Ag8403|
|Full Name||NAD(P)H dehydrogenase, quinone 2|
|Calculated molecular weight||231 aa, 26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC006096|
|Gene ID (NCBI)||4835|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.
|Product Specific Protocols|
|WB protocol for NQO2 antibody 15767-1-AP||Download protocol|
|IHC protocol for NQO2 antibody 15767-1-AP||Download protocol|
|IF protocol for NQO2 antibody 15767-1-AP||Download protocol|
|IP protocol for NQO2 antibody 15767-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Physiol Biochem
Downregulation of quinone reductase 2 attenuates vascular smooth muscle cells proliferation and neointimal formation in balloon injured rat carotid artery.
J Exp Clin Cancer Res
PRX1 knockdown potentiates vitamin K3 toxicity in cancer cells: a potential new therapeutic perspective for an old drug.
Apigenin and Luteolin Regulate Autophagy by Targeting NRH-Quinone Oxidoreductase 2 in Liver Cells.